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Plasmid#62938PurposeExpression of (+36)GFP-GGS9-Cre-6xHis in bacterial cellsDepositorInsert(ps36)GFP-9xGGS-Cre
UseTags(pos36)GFP and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-GFP-Patronin CC 868–1457
Plasmid#59053Purposefor E. coli expression of Patronin CC truncationDepositorInsertPatronin (Patronin Fly)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CTLA4-FLN-ELP-His-ybbr
Plasmid#168038PurposeExpresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertCytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
UseTags3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET mRuby2 LIC cloning vector (H6-mRuby2)
Plasmid#86934PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2DepositorTypeEmpty backboneUseTagsBiotin, His6, Tev Recognition Sequence, and mRuby…ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-GST-eIF4G1-His6, eIF4E
Plasmid#37232DepositorUseTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET tdTomato LIC cloning vector (H6-tdTomato)
Plasmid#86928PurposepET LIC cloning vector for Biotin-His6-tev-yORF-tdTomatoDepositorTypeEmpty backboneUseTagsBiotin, His6, Tev Recognition Sequence, and tdTom…ExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-OPTN WT
Plasmid#190191PurposePlasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797DepositorInsertoptineurin (OPTN Human)
UseTags6xHis, TEV, and mCherryExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)
Plasmid#182395PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusionsDepositorInsertsVNp-LZ-VenusN154
VNp-LZ-VenusC155
UseTagsVesicle Nucleating peptide (VNp) & Leucine Zi…ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
FabF (KASII) pet28 N-terminal His Tag
Plasmid#75017PurposeExpresses the ketosynthase FabF/KASII from the E. coli fatty acid synthaseDepositorInsertFabF (fabF E. coli)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PET-SpCas9-HF1-plus-NLS-6xHis
Plasmid#207381PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSpCas9-HF1-plus
UseCRISPRTags6xHisExpressionBacterialMutationN497A, Q695A, Q926A, amino acids 1005-1013 replac…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET mClover LIC cloning vector (H6-mClover)
Plasmid#86931PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mCloverDepositorTypeEmpty backboneUseTagsBiotin, His6, Tev Recognition Sequence, and mClov…ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b – E. coli RecE residues 564-868
Plasmid#104534PurposeExpresses E. coli RecE residues 564-868DepositorInsertRecE residues 564-866 (C-terminal nuclease domain)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
Plasmid#167849PurposeBacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNADepositorInsertnsp10, nsp16
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
HOXD8 (human,partial) HIS-tag pET
Plasmid#8569DepositorInsertHOXD8 (HOXD8 Human)
UseTagsHis and T7ExpressionBacterialMutationPartial protein containing 4 AA N-terminal to HD …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET mClover LIC cloning vector (u-mClover)
Plasmid#86929PurposepET LIC cloning vector for yORF-mCloverDepositorTypeEmpty backboneUseTagsmCloverExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-Aurein1.2_(+36GFP)-LPETG-His
Plasmid#71755PurposeHis-TEV-Aurein1.2+36GFP-LPETG-His expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-LPETG-His
UseTags6XHis, Sortase (LPETG), and TEV ProteaseExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
3159_pETcon-SARS-CoV-2-RBD-L452R-T478K
Plasmid#184407Purposeyeast surface display of the SARS-CoV-2 Delta variant RBDDepositorInsertSARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationL452R+T478KPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-32M human DKK1c (183–266)
Plasmid#132626Purposeexpresses human DKK1c (183–266) in E. coliDepositorInsertDKK1 (183–266)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET LIC cloning vector for hairpin mRNA (2U-T)
Plasmid#29719DepositorTypeEmpty backboneUseTagsMYFQSNAExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2011AvailabilityAcademic Institutions and Nonprofits only