-
Plasmid#135185PurposeTPC2 with truncated N-terminus, tagged with mCherry on C terminus.DepositorInsertTPC2 (Tpcn2 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationDeleted amino acids 1-49PromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TCbt
Plasmid#44515DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
GGH-bio-His
Plasmid#52066PurposeExpresses full-length Gamma-glutamyl hydrolase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertGGH (GGH Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmThor-V5His6
Plasmid#79408PurposeExpresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 cellsDepositorInsertDmThor (Thor Fly)
UseSchneider 2 cell expressionTagsEGFP and V5-His6ExpressionMutationPromoterAc5 promoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX62
Plasmid#46854PurposeTOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCEDepositorInsertattB-Puro-2A and Hupki p53 G245S (TP53 Human, Mouse)
UseTagsExpressionMammalianMutationG245SPromoterAvailable sinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
F2RL3-bio-His
Plasmid#51864PurposeExpresses full-length Proteinase-activated receptor 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertF2RL3 (F2RL3 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K5S
Plasmid#34598DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationFragment 1781-2086 of KLF4PromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe EGFR Del5(T790M)
Plasmid#32069DepositorInsertEGFR (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationDelete E746-S752, insert V, T790MPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1GZmbh
Plasmid#44516DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
His 3C Kinase dead MKK6 1-334
Plasmid#87882PurposeE. coli expression of MKK6 kinase deadDepositorInsertMKK6 (MAP2K6 Human)
UseTags3C protease site and 6xHisExpressionBacterialMutationK82A, kinase dead mutantPromoterT7Available sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADSL-2XStrep
Plasmid#125684Purposeexpresses human ADSL protein with a C-terminal 2XStrep affinity tagDepositorInsertADSL (ADSL Human)
UseTags2X Strep-Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
BAMBI-bio-His
Plasmid#51866PurposeExpresses full-length Basigin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertBAMBI (BAMBI Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJD-KLF4-ER
Plasmid#34596DepositorInsertKLF4 (KLF4 Human)
UseRetroviralTagsMyc epitope tag and estrogen receptor fusionExpressionMammalianMutationPromoterLTRAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST-EGFP-Cx43ML-N1
Plasmid#49860PurposeEncodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tagDepositorInsertConnexin 43 (GJA1 Human)
UseTagsEGFPExpressionMammalianMutationAll internal Methionines mutated to Leucine. No s…PromoterCMVAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1mLCAD_K42R_FLAG
Plasmid#24486DepositorInsertLong chain acyl-CoA dehydrogenase (Acadl Mouse)
UseTagsFLAGExpressionMammalianMutationLys42ArgPromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-ML-HA
Plasmid#49857PurposeEncodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tagDepositorInsertConnexin 43 (GJA1 Human)
UseTagsHAExpressionMammalianMutationAll internal Methionines mutated to Leucine. Cont…PromoterCMVAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH744-CEN-RLuc/slowmaxCFLuc
Plasmid#38224DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3 (=GPD)Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail
Plasmid#60392PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoterDepositorInsertTUB1 (TUB1 Synthetic, Budding Yeast)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
FAM-bio-His
Plasmid#51874PurposeExpresses full-length Protein FAM171A1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertFAM (FAM171A1 Synthetic, Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-NoStart
Plasmid#49853PurposeEncodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationStart codon mutated to from methionine to isoleuc…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only