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Plasmid#139683PurposeNup62 dimerization construct fused to 2 copies of GFP under an CMV promoterDepositorInsertNup62 (NUP62 Human)
UseLentiviralTags2 copies of GFP and Dimerization domain FKBPExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC007 - pRFP constitutive expression
Plasmid#79156PurposeRFP constitutively expressedDepositorInsertRFP
UseTagsExpressionMutationPromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA flag PPAR gamma
Plasmid#8895PurposeMammalian expression of flag-tagged PPAR gammaDepositorInsertPPAR gamma (Pparg Mouse)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαq*-BERKY3
Plasmid#158217PurposeBRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gα13*-BERKY3
Plasmid#158218PurposeBRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-YHRC
Plasmid#54336PurposeExpression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.DepositorTypeEmpty backboneUseTags6His-SUMO (yeast gene: SMT3)ExpressionBacterialMutationPromoterT7-LacOAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 wt
Plasmid#80876Purposemammalian expression of ALK5 WTDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro
Plasmid#138152PurposeExpresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleusDepositorInserteGFP-MCS
UseLentiviralTags2PEST AND NLSExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-CaM
Plasmid#127393PurposeFluorescent reporter for calmodulin 1DepositorInsertCalmodulin 1 (Calm1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterpCAGAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P13
Plasmid#120024PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherryDepositorInsertmCherry
UsePcr templateTagsmCherryExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-GFP SNAP25
Plasmid#170872PurposeMammalian expression of His-GFP SNAP25.DepositorInsertSNAP 25 (Snap25 Rat)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pUC57-CmYLCV
Plasmid#205245PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)
Plasmid#179005PurposeSp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)
Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 WT
Plasmid#12301DepositorInsertSTAT1 (STAT1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_mCherry
Plasmid#182939Purposealpha7 nACHR expression in mammalian cell linesDepositorInsertNACH7 (CHRNA7 Human)
UseSynthesizedTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mRuby3
Plasmid#155221PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z21
Plasmid#120064PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTagDepositorInsert24xSunTag
UsePcr templateTags24xSunTagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only