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pMXs-hs-HRAS

Plasmid

#52730

Purpose

retroviral expression of human HRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

HRAS (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
ELMOD3

Plasmid

#155584

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ELMOD3 (ELMOD3 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DHX40

Plasmid

#155540

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DHX40 (DHX40 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CDC40

Plasmid

#155444

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CDC40 (CDC40 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CCNL2

Plasmid

#155441

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CCNL2 (CCNL2 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CCNL1

Plasmid

#155440

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CCNL1 (CCNL1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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BTG3

Plasmid

#155427

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

BTG3 (BTG3 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EXOSC2

Plasmid

#155590

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

EXOSC2 (EXOSC2 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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LUC7L

Plasmid

#155713

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

LUC7L (LUC7L Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (del.Cyto)

Plasmid

#49209

Purpose

expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (del.Cyto) (Havcr2 Mouse)

Use

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

cytosolic domain deleted, no Tim3 signal sequence…

Promoter

Ef1a

Available since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pLX304-PCDH7-c

Plasmid

#108540

Purpose

Expresses human PCDH7 transcript variant c in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant c (PCDH7 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304-PCDH7-b

Plasmid

#108539

Purpose

Expresses human PCDH7 transcript variant b in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant b (PCDH7 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMG035 MBP-Axin∆BCD-His

Plasmid

#154053

Purpose

Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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mVenus-proα2(I)

Plasmid

#119838

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-3F2-mCherry

Plasmid

#73418

Purpose

Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Promoter 3F2 (orthogonal T7-lac variant)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P3F2 (orthogonal T7-lac variant)

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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EFNB2-tdTomato

Plasmid

#139967

Purpose

Donor vector to knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

Human EFNB2 knockin homology arms (EFNB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL3-0455

Plasmid

#115463

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-0455 (MBNL3 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

Plasmid

#171102

Purpose

CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9 (Otx2 S. pyogenes, Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBS L30 Ruby3-Rab7A

Plasmid

#135651

Purpose

Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab7A (RAB7A Human)

Use

Tags

Ruby3

Expression

Mammalian

Mutation

Promoter

L30

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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tdEos-Actin-C-18

Plasmid

#57571

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Actin (ACTB Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits