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pEB1deltaC-2xEGFP shRNA resistant

Plasmid

#37925

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion after aa248, TTCCTGT=>TCCCGGT

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-somBiPOLES-eGFP

Plasmid

#192584

Purpose

AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

human synapsin

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPC2-gephyrin S268A/270A

Plasmid

#199608

Purpose

Encodes N-terminal eGFP-tagged P1-gephyrin S268/270A for expression in mammalian cells.

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S268A/S270A

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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peAAV-CKII(0.4)-Chronos_eGFP_GP2A_nls_mScarlet

Plasmid

#121542

Purpose

Chronos with red nucleus-targeted fluorescence for excitatory neurons

Depositor

Matthias Prigge

Insert

blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

CamKII

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p156 pWZL-EGFP-SLPI

Plasmid

#11069

Depositor

Ramesh Shivdasani

Article

Schulze et al Blood. 2004 Dec 15. 104(13):3949-57.

Insert

SLPI (Slpi Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pPLN-hFluc-DM-eGFP-KDEL

Plasmid

#177728

Purpose

DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFlucDM

Use

Tags

KDEL, Prolactin Signal Sequence, and eGFP

Expression

Bacterial and Mammalian

Mutation

R188Q, R261Q (Destabilized Mutant)

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPC3/GgVcl 884-1066 (Vt)

Plasmid

#46272

Depositor

Susan Craig

Insert

Vcl 884-1066 (Vt) (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

884-1066 (Vt) tail domain

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo5(N140A)

Plasmid

#157806

Purpose

Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo5(N140A)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

N140A

Promoter

pTAC

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-TK1

Plasmid

#161919

Purpose

Expresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Thymidine kinase, cytosolic (TK1 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN E50K

Plasmid

#175765

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN E50K

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Tags

Expression

Mutation

E50K

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCasper-pNos-PCP-eGFP

Plasmid

#180274

Purpose

to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Article

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

PCP-eGFP

Use

Tags

eGFP

Expression

Insect

Mutation

Promoter

nanos

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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iLID-eGFP-2xC2-iLID

Plasmid

#114418

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKC518 pBABE EGFP-LIN52

Plasmid

#192697

Purpose

Retroviral transgene expression of human LIN52

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

LIN52 (LIN52 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-eGFP

Plasmid

#117794

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBSU6-shScr/CMV-eGFP

Plasmid

#89912

Purpose

Express shRNA Scrambled control

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Scrambled

Use

Tags

eGFP under separate promoter

Expression

Mammalian

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBS IRES EGFP (DM#271)

Plasmid

#15080

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

IRES EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-RapR-FAK-YM-Y397F

Plasmid

#25930

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-FAK (Ptk2 Human, Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pEBB-II-I-EGFP delta isoform

Plasmid

#22149

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 A114Q

Plasmid

#175767

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 A114Q

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

A114Q

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-EGFP

Plasmid

#164940

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Expression

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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