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Plasmid#99935Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationdelAA153-165insNAAIRS linkerPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 N179-193 (#437)
Plasmid#99936Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationdelAA179-193insNAAIRS linkerPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 N207-225 (#439)
Plasmid#99938Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationdelAA207-225insNAAIRS linkerPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_K5S_S1966C
Plasmid#98683PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S and S1966CDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationK1838S, K1859S, K1866S, K1873S, K1887S, S1966CPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 N109-119 (#432)
Plasmid#99933Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationdelAA109-119insNAAIRS linkerPromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 1-165 (#187)
Plasmid#99924Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationC-terminal deletion; encodes AA 1-165PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-664
Plasmid#92252PurposePlasmid for expression of GST-tagged human TNRC6C CED with mutated PAM2 motif in mammalian cellsDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsGSTExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterEF1AAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-658
Plasmid#92257PurposePlasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motifDepositorInsertTNRC6C CED (TNRC6C Human)
UseTagsNHAExpressionMammalianMutationC-terminal effector domain (CED): aa 1369-1690 wi…PromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_SCP1 vector_blocking 2
Plasmid#99317PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoK-OG241
Plasmid#72840PurposeNOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.DepositorInsertPatoK
UseTagsExpressionBacterialMutationPromoterPatoKAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoL-LOG241
Plasmid#72846PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.DepositorInsertPatoL
UseTagsExpressionBacterialMutationPromoterPatoLAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoK-LOG241
Plasmid#72848PurposeNOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.DepositorInsertPatoK
UseTagsExpressionBacterialMutationPromoterPatoKAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pClneoEGFPC-let60 K16N
Plasmid#66861PurposeExpress GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cellsDepositorInsertLet-60 (let-60 Nematode)
UseTagsGFPExpressionMammalianMutationLysine 16 is mutated to asparaginePromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A
Plasmid#65660PurposeFLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged glutamic acid 97 to alanine, lysine 100 t…PromoterCMV, 2x TetAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)
Plasmid#52898PurposeYpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.DepositorInsertYpd*1-L-IFP-F[2]
UseTagsL-IFP-F[2]ExpressionYeastMutationChanged Tryptophane 80 to AlaninePromoterGAL1Available sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-NtermN3
Plasmid#51448PurposeExpresses TALEN targeting left CCR5A site (L18) with N3 N-terminal domainDepositorInsertTALEN CCR5 Left (L18) N3 N-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK150Q, K153Q, and R154QPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-NtermN3
Plasmid#51449PurposeExpresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domainDepositorInsertTALEN CCR5 Right (R18) N3 N-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK150Q, K153Q, and R154QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBG MKK3b (Glu)
Plasmid#50451Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
UseTagsGstExpressionMammalianMutationchanged Serine 218 to Glutamic Acid, changed Thre…PromoterSV40Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
1287 p3E-mut let-7 etv2 3’ UTR
Plasmid#49011PurposeZebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vectorDepositorInsertets variant gene 2 (etsrp Zebrafish)
UseGateway 3' entryTagsExpressionMutationMutated the Let7 miRNA binding sitesPromoterAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFM038
Plasmid#40141DepositorInsertpar-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationR183-5A + 7SA; base pairs 6-523 of par-2 were re…Promoterpie-1Available sinceOct. 23, 2012AvailabilityAcademic Institutions and Nonprofits only