Addgene

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A3Bctd-max

Plasmid

#198888

Purpose

Express A3Bctd-max BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)

Use

Tags

6x His and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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A3Bmax

Plasmid

#207167

Purpose

Express full-length human A3B in a BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)

Use

Tags

NLS and NLS, 6x His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TXNIP-py-FLAG

Plasmid

#200092

Purpose

TXNIP py motif mutant with C-terminal 3xFLAG tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

TXNIP (TXNIP Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

py motifs mutated (aa331-334 and 375-378)

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-DmCCR4_1-166_R

Plasmid

#147439

Purpose

Bacterial Expression of DmCCR4_1-166

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_1-166 (twin Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB9339 (pgRNA_VII-1_NatMX)

Plasmid

#161591

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9340 (pgRNA_VIII-1_NatMX)

Plasmid

#161592

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9342 (pgRNA_XIII-1_NatMX)

Plasmid

#161594

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9344 (pgRNA_XVI-1_NatMX)

Plasmid

#161596

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-14

Plasmid

#160714

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8

Plasmid

#175796

Purpose

de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8_SS

Plasmid

#175798

Purpose

de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing disulfide 'staples'

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8_SS

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8_SH2sub6

Plasmid

#175799

Purpose

de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing 2 copies of human Nck2 SH2

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8_SH2sub6

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP9sub3

Plasmid

#175794

Purpose

de novo thick circular tandem repeat protein trimer formed from 3-repeat monomers

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP9sub3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub6

Plasmid

#175795

Purpose

de novo thick circular tandem repeat protein tetramer formed from 6-repeat monomers

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub6

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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Mdn1-deltaC

Plasmid

#171603

Purpose

Expresses S. pombe Mdn1 a.a. 1-3911 in insect cells.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

Mdn1

Use

Tags

His6 tag

Expression

Insect

Mutation

C-terminal deletion; a.a. 1-3911 only

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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Ash1

Plasmid

#169711

Purpose

Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)

Depositor

Tanja Mittag

Article

Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

Insert

Ash1 (ASH1 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Truncation including encoding amino acids 420-500

Promoter

T7

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-17

Plasmid

#160717

Purpose

TrpC Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites

Use

Synthetic Biology

Tags

Expression

Mutation

Incorporation of BsaI restriction sites

Promoter

Available since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-15

Plasmid

#160715

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-FLAG peptide

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-3

Plasmid

#160703

Purpose

tef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tef1-alpha

Use

Synthetic Biology

Tags

Expression

Mutation

Incorporation of BsaI restriction sites

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pZA8

Plasmid

#158486

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Tags

Expression

Mutation

L17E, D481V, STOP codon

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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