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Plasmid#132710PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh geneDepositorInsertshCrh(2) (Crh Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-AuroraB L154A/H250Y
Plasmid#108491Purposeexpression of EGFP-AuroraB Analog sensitive (L154A/H250Y)DepositorInsertAuroraB (AURKB Human)
UseTagsEGFPExpressionMammalianMutationAnalog sensitive L154A/H250YPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Tet-on-vsv-Incenp d543-746iPRC1_279-482-GFP
Plasmid#108500Purposeinducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFPDepositorInsertINCENP d543-746iPRC1 304-509 (PRC1 Human)
UseTagsEGFP and VSVExpressionMammalianMutationdelta alpha Helix, insert PRC1 microtubule domainPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP
Plasmid#108503Purposeinducible expression of vsv-INCENP d543-746-EGFPDepositorInsertINCENP d543-746 (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationdelta alpha helixPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp d543-746iPRC1_279-482-GFP
Plasmid#108504Purposeinducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFPDepositorInsertINCENP d543-746iPRC1 304-509 (PRC1 Human)
UseTagsEGFP and VSVExpressionMammalianMutationdelta alpha Helix, insert PRC1 microtubule domainPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp 8KR-GFP
Plasmid#108505Purposeinducible expression of vsv-INCENP 8KR-EGFPDepositorInsertINCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationK548R, K563R, K602R, K609R, K614R, K622R, K630R, …PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-vsv-Incenp 8KQ-GFP
Plasmid#108506Purposeinducible expression of vsv-INCENP 8KQ-EGFPDepositorInsertINCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationK548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
vsv-Incenp 1-48-GFP
Plasmid#108507Purposeexpression of vsv-INCENP 1-48-EGFPDepositorInsertCEN-Box of INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationonly CEN box of INCENPPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
vsv-Incenp 1-58-GFP
Plasmid#108508Purposeexpression of vsv-INCENP 1-58-EGFPDepositorInsertCEN-Box of INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationonly CEN box of INCENPPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
vsv-Incenp 1-63-GFP
Plasmid#108509Purposeexpression of vsv-INCENP 1-63-EGFPDepositorInsertCEN-Box of INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationonly CEN box of INCENPPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-INCENP d543-746
Plasmid#108473Purposeexpression of vsv-INCENP d543-746DepositorInsertINCENP d543-746 AA (INCENP Human)
UseTagsVSVExpressionMammalianMutationDelta Alpha helix, aa543-746 deletedPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-AuroraB L154A/H250Y
Plasmid#108489Purposeexpression of vsv-AuroraB Analog sensitive (L154A/H250Y)DepositorInsertAuroraB (AURKB Human)
UseTagsVSVExpressionMammalianMutationAnalog sensitive L154A/H250YPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
hASXL(1-115)-luciferase
Plasmid#106427PurposeExpresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferaseDepositorInsertaddition sex combs like 1 ASXL1 (ASXL1 Human)
UseTagsluciferaseExpressionMammalianMutationamino acids 1-115 fused to luciferasePromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SPA mimic WT
Plasmid#92350PurposeTo mimic SPA RNA processing and expressionDepositorInsertSNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR CMVie-Lifeact-EGFP L1-R5
Plasmid#84391PurposeMultisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGatewayTagsEGFPExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
KS6A1_HUMAN_D1
Plasmid#79720PurposeThis plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKS6A1 (RPS6KA1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1G3_HUMAN_D0
Plasmid#79685PurposeThis plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1G3 (CSNK1G3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His
Plasmid#83458PurposeHuman PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)DepositorInsertPolycystin-1 (PKD1 Human)
UseTags6xHis and MycExpressionMammalianMutationAmino-acids 4127-4303, S4144DPromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT
Plasmid#61789PurposeLuciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutatedDepositorInsertmouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
UseLuciferaseTagsExpressionMutationboth miR-200c MRE-2 and MRE-3 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-1D4-mCherry
Plasmid#73423PurposeReporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.DepositorInsertPromoter 1D4 (orthogonal T7-lac variant)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterP1D4 (orthogonal T7-lac variant)Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only