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Plasmid#148516PurposeBacterial Expression of DmThor_50-83-P66KP71QDepositorInsertDmThor_50-83-P66KP71Q (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor-del76-84_Z
Plasmid#148190PurposeBacterial Expression of DmThor-del76-84DepositorInsertDmThor-del76-84 (Thor Fly)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvM-DmThor-del76-84-Y54AM59A_Z
Plasmid#148191PurposeBacterial Expression of DmThor-del76-84-Y54AM59ADepositorInsertDmThor-del76-84-Y54AM59A (Thor Fly)
UseTagsExpressionBacterialMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-CLL78AAA-V5His6_Z
Plasmid#148165PurposeInsect Expression of DmThor-CLL78AAADepositorInsertDmThor-CLL78AAA (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-RGTtoAAA-V5His6_Z
Plasmid#148166PurposeInsect Expression of DmThor-RGTtoAAADepositorInsertDmThor-RGTtoAAA (Thor Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-Y54AM59A-V5His6_Z
Plasmid#148167PurposeInsect Expression of DmThor-Y54AM59ADepositorInsertDmThor-Y54AM59A (Thor Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-del76-84-V5His6_Z
Plasmid#148152PurposeInsect Expression of DmThor-del76-84DepositorInsertDmThor-del76-84 (Thor Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-del76-85-V5His6_T
Plasmid#147613PurposeInsect Expression of DmThor-del76-85DepositorInsertDmThor-del76-85 (Thor Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HseIF4G_571-676-GB1_AG
Plasmid#148745PurposeBacterial Expression of HseIF4G_571-676DepositorInsertHseIF4G_571-676 (EIF4G1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HseIF4G_592-653_AG
Plasmid#148746PurposeBacterial Expression of HseIF4G_592-653DepositorInsertHseIF4G_592-653 (EIF4G1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-β-catenin_S23A,T40A,T41A,T112A
Plasmid#190824PurposeFor mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.DepositorInsertβ-catenin (CTNNB1 Chicken)
UseTagsGFPExpressionMammalianMutationS23A, T40A, T41A, T112APromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676-GB1_AD
Plasmid#148512PurposeBacterial Expression of DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676DepositorInsertDmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-5Flnc
Plasmid#174870PurposeCRISPR vector for generating Flnc STREAMING-tag KI cellDepositorInsertsgRNA for mouse Flnc (Flnc Synthetic)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free PcPTS
Plasmid#182483PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
PTS
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (AG4TGGA)2
Plasmid#182488PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free (PT)4P
Plasmid#182490PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-Del-1
Plasmid#190095Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR delete-1 (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationdeleted the base pairs 6704-6709 refer to NM_0070…PromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-Del-2
Plasmid#190096Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR delete-2 (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationdeleted the base pairs 7122-7129 refer to NM_0070…PromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pT7-EGFP-C1-HseIF4G1_615-654_AD
Plasmid#148518PurposeMammalian Expression of HseIF4G1_615-654DepositorInsertHseIF4G1_615-654 (EIF4G1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only