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Plasmid#212044PurposeLR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only
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pcDNA5-FRT-TO-NUbo-E3(48)-FLAG
Plasmid#212802PurposeConstitutive/Doxycycline-inducible expression of NUbo-E3(48)-FLAG in mammalian cellsDepositorInsertE3(48)
UseTagsFLAG and NUboExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24-95~236
Plasmid#208634Purposefor E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236DepositorInserthuman RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationonly aa 95~236, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(R150A)-3xFLAG-pCDNA
Plasmid#201966PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationR150APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-Δ220-234-Flag
Plasmid#210341PurposeExpresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationΔ220-234PromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-H120N/F121L-Flag
Plasmid#210340PurposeExpresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationH120N/F121LPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Qt-FLAG-TGA-rt9us-antiGFP
Plasmid#206954PurposeMammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)DepositorInsertQtEnc, antiGFP-intrabody
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLuc/FLAG Metap2
Plasmid#209316PurposeLentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)DepositorInsertMETAP2 (METAP2 Human, human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLYCHOS/LYCHOS WT-FLAG
Plasmid#209315PurposeLentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)DepositorInsertLYCHOS (GPR155 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO shLYCHOS/FLAG Metap2
Plasmid#209314PurposeLentiviral construct expressing FLAG Metap2 and shRNA against LYCHOSDepositorInsertLYCHOS (GPR155 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Mts D85N
Plasmid#208403PurposeExpresses FLAG-tagged Drosophila mts protein with D85N mutationDepositorInsertmicrotubule star (mts Fly)
UseTags3xFLAGExpressionInsectMutationD85NPromoterUbi-p63eAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS7 puro
Plasmid#208405PurposeExpresses FLAG-tagged Drosophila IntS7 from inducible MtnA promoterDepositorInsertpMT FLAG MCS puro (defl Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS13 puro
Plasmid#208406PurposeExpresses FLAG-tagged Drosophila IntS13 from inducible MtnA promoterDepositorInsertIntS13 (asun Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMtnA FLAG-IntS14 puro
Plasmid#208407PurposeExpresses FLAG-tagged Drosophila IntS14 from inducible MtnA promoterDepositorInsertIntS14 (IntS14 Fly)
UseTags3xFLAGExpressionInsectMutationPromoterMtnAAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Mts H59Q
Plasmid#208402PurposeExpresses FLAG-tagged Drosophila mts protein with H59Q mutationDepositorInsertmicrotubule star (mts Fly)
UseTags3xFLAGExpressionInsectMutationH59QPromoterUbi-p63eAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Mts H241A
Plasmid#208404PurposeExpresses FLAG-tagged Drosophila mts protein with H241A mutationDepositorInsertmicrotubule star (mts Fly)
UseTags3xFLAGExpressionInsectMutationH241APromoterUbi-p63eAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3 APPA
Plasmid#207124PurposeLentiviral plasmid to express mouse Mettl3 APPA mutantDepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HAExpressionMutationD395A and W398APromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-3xFLAG-LMNB1
Plasmid#207777PurposeDonor template for Blast-2A-3xFLAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorArticleInsertLMNB1 Homology Arms flanking a Blast-3xFLAG Cassette (LMNB1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUb FLAG-Zebrafish IntS6
Plasmid#198410PurposeExpresses FLAG-tagged zebrafish IntS6DepositorInsertints6 (ints6 Zebrafish)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Zebrafish IntS6L
Plasmid#198411PurposeExpresses FLAG-tagged zebrafish IntS6LDepositorInsertints6l (ints6l Zebrafish)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only