Addgene

We narrowed to 4,148 results for: 027

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SPOP-V5 (BACK))

Plasmid

#169722

Purpose

Mammalian Expression of mouse SPOP Y353E

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Y353E

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcold-6xHis-HRV3Csite-DmLoqsPD

Plasmid

#41095

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PD (loqs Fly)

Use

Tags

6xHis-HRV3Csite

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pcold-6xHis-HRV3Csite-DmLoqsPB

Plasmid

#41094

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PB (loqs Fly)

Use

Tags

6xHis-HRV3Csite

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pGEX4T-Mst1 SARAH

Plasmid

#84297

Purpose

Expresses Mst1 SARAH domain for interaction studies.

Depositor

Jonathan Chernoff

Article

Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

Insert

Mst1 (STK4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pILGFP84

Plasmid

#83542

Purpose

Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSA1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

SSA1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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MTK234_055

Plasmid

#123920

Purpose

Encodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

gRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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UKNP1.19.1

Plasmid

#98192

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.20.1

Plasmid

#98195

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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mVenus-PKCB1 K371R

Plasmid

#195230

Purpose

Retroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PKCB1 (PRKCB Human)

Use

Retroviral

Tags

Expression

Mutation

K371R

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PKCBII

Plasmid

#195232

Purpose

Mammalian expression vector containing GFP tagged PKCBII.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PKCB2 (PRKCB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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PDGFR-TMD (MTK 3B) (pZYW025)

Plasmid

#194218

Purpose

Mammalian Toolkit part 3b encoding the transmembrane domain of PDGFR

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PDGFR TMD

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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T2A-mCherry (MTK 4a) (pAN3844)

Plasmid

#194223

Purpose

Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

T2A-mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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A3Bctd-max

Plasmid

#198888

Purpose

Express A3Bctd-max BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)

Use

Tags

6x His and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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A3Bmax

Plasmid

#207167

Purpose

Express full-length human A3B in a BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)

Use

Tags

NLS and NLS, 6x His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TXNIP-py-FLAG

Plasmid

#200092

Purpose

TXNIP py motif mutant with C-terminal 3xFLAG tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

TXNIP (TXNIP Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

py motifs mutated (aa331-334 and 375-378)

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-DmCCR4_1-166_R

Plasmid

#147439

Purpose

Bacterial Expression of DmCCR4_1-166

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_1-166 (twin Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB9339 (pgRNA_VII-1_NatMX)

Plasmid

#161591

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9340 (pgRNA_VIII-1_NatMX)

Plasmid

#161592

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site VIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9342 (pgRNA_XIII-1_NatMX)

Plasmid

#161594

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9344 (pgRNA_XVI-1_NatMX)

Plasmid

#161596

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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