Skip to main content
Addgene
Showing: 9101 - 9150 of 16283 results

  2. pET28a(+)-HisTim12_CtoA

    Plasmid
    #170281
    Purpose
    Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2
    Insert
    yeast Tim12 (TIM12 Budding Yeast)
    Use
    Tags
    TEV-cleavable N-terminal His tag (on backbone)
    Expression
    Bacterial
    Mutation
    mutated two non-conserved Cys to Ala
    Promoter
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170281-plasmid-map-sequence-id-334955

    Enlarge

  3. pCDNA3_PKA-CαN (E12,14A)-mVenus

    Plasmid
    #168499
    Purpose
    Mammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168499-plasmid-map-sequence-id-332636

    Enlarge

  4. pCDNA3_PKA-CαN (K8,9R)-mVenus

    Plasmid
    #168498
    Purpose
    Mammalian expression of the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168498-plasmid-map-sequence-id-332635

    Enlarge

  5. pCDNA3_PKA-CαN(K8,9A)-mPAGFP

    Plasmid
    #168494
    Purpose
    Mammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168494-plasmid-map-sequence-id-332608

    Enlarge

  6. pCDNA3_PKA-CαN (E12,14K)-mVenus

    Plasmid
    #168488
    Purpose
    Mammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168488-plasmid-map-sequence-id-332541

    Enlarge

  7. pCDNA3_PKA-CαN (K8,9E)-mVenus

    Plasmid
    #168487
    Purpose
    Mammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168487-plasmid-map-sequence-id-332540

    Enlarge

  8. pCDNA3_PKA-CαN (K22,24A)-mVenus

    Plasmid
    #168484
    Purpose
    Mammalian expression of the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168484-plasmid-map-sequence-id-332537

    Enlarge

  9. pCDNA3_PKA-CαN (K8,9A)-mVenus

    Plasmid
    #168483
    Purpose
    Mammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168483-plasmid-map-sequence-id-332536

    Enlarge

  10. pCDNA3_PKA-CαN (G2A)-mVenus

    Plasmid
    #168482
    Purpose
    Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
    Depositor
    Haining Zhong
    Article
    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11
    Insert
    the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    168482-plasmid-map-sequence-id-332531

    Enlarge

  11. C513-E06: attB5r-pA-polyhedrinp>-attB1r

    Plasmid
    #162948
    Purpose
    Gateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectors
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    AcMNPV polyhedrin promoter with upstream polyA sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162948-plasmid-map-sequence-id-330994

    Enlarge

  12. C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

    Plasmid
    #162965
    Purpose
    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    His6-Halotag fusion tag with upstream E. coli ribosome binding site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    162965-plasmid-map-sequence-id-330946

    Enlarge

  13. C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

    Plasmid
    #162964
    Purpose
    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    162964-plasmid-map-sequence-id-330937

    Enlarge

  17. C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r

    Plasmid
    #162934
    Purpose
    Gateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoter
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    His6-glutathione-S-transferase fusion, AcMNPV polyhedrin promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162934-plasmid-map-sequence-id-330542

    Enlarge

  18. C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r

    Plasmid
    #162933
    Purpose
    Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    His6-maltose binding protein fusion, AcMNPV polyhedrin promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162933-plasmid-map-sequence-id-330541

    Enlarge

  25. pCAG-mEGFP-HO-G

    Plasmid
    #164163
    Purpose
    Expresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminal
    Depositor
    Isei Tanida
    Article
    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1
    Insert
    mEGFP A236K
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    164163-plasmid-map-sequence-id-323957

    Enlarge

  35. FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k

    Plasmid
    #127403
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: K295 to Amber codon, W340A,…
    Promoter
    CMV
    Available since
    July 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    127403-plasmid-map-sequence-id-248720

    Enlarge

  36. FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k

    Plasmid
    #127407
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: E322 to Amber codon, K305H,…
    Promoter
    CMV
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127407-plasmid-map-sequence-id-247970

    Enlarge

  37. FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k

    Plasmid
    #127401
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: K295 to Amber codon, W340A,…
    Promoter
    CMV
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127401-plasmid-map-sequence-id-247963

    Enlarge

  39. pESC/mCitrine/erg3-mCherry

    Plasmid
    #117993
    Purpose
    Co-expression of mCitrine under the control of the Gal1 promoter and erg3 fused to mCherry (an ER-targeting marker protein) under the control of the Gal10 promoter
    Depositor
    Mathias Pribil
    Article
    Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102
    Insert
    erg3-mCherry
    Use
    Plant/bacteria binary vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Gal10
    Available since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    117993-plasmid-map-sequence-id-237531

    Enlarge

  40. pAL928

    Plasmid
    #119853
    Purpose
    precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Cysteine 169 to Serine, Cysteine 179 to Serine, C…
    Promoter
    T7
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119853-plasmid-map-sequence-id-232052

    Enlarge

  45. pBV163

    Plasmid
    #108364
    Purpose
    Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.
    Depositor
    Scott Moye-Rowley
    Article
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    Knock-in Cg TDH3 promoter in Cg YAP1 gene
    Use
    Replace c.glabrata yap1 promoter with c. glabrata…
    Tags
    Expression
    Mutation
    Promoter
    TDH3
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108364-plasmid-map-sequence-id-209913

    Enlarge

  49. GST-prRDH-CTS

    Plasmid
    #86069
    Purpose
    A bacterial expression plasmid encoding photoreceptor retinol dehydrogenase ciliary targeting signal with N-terminus GST-tag.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Retinol dehydrogenase (RDH8 Bovine)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    the insert is 297-312 of RDH8 (Bos taurus)
    Promoter
    tac
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86069-plasmid-map-sequence-id-177979

    Enlarge

Showing: 9101 - 9150 of 16283 results