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Plasmid#138566PurposeExpresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationL1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H2A-TORCAR
Plasmid#175016PurposeTORCAR targeted to the nucleus via histone H2A.DepositorInsertH2A-TORCAR
UseTagsHistone H2AExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hArfaptin1
Plasmid#206404PurposeMammalian expression of hArfaptin1DepositorInsertArfaptin1 (ARFIP1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAPK13
Plasmid#23739DepositorInsertMAPK13 (MAPK13 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseTagsExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKD3_mCherry
Plasmid#187385PurposeTo chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRTDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
4xCSL-luciferase
Plasmid#41726DepositorInsert4xCSL binding sites
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationContains a multimerized high affinity CSL binding…PromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRiA4-VIR
Plasmid#138193PurposeVirulence helper compatible with pCambia binary vectors.DepositorInsertvir gene cluster
UseVir gene expressionTagsExpressionMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL-TRBO
Plasmid#80082PurposeAn improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS-sfGFP
Plasmid#117390PurposeTn7 tagging vector pTn7xTS with sfGFP expression scaffoldDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtacAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDV05.0_pEF1a-iRFP720
Plasmid#200407PurposeExpresses iRFP720 constitutively in mammalian cellsDepositorInsertiRFP720
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKL09-GCaMP6f-mScarlet bb118
Plasmid#158979PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.DepositorInsertGCaMP6f-mScarlet fusion
UseTagsExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23118 (sigma 70)Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3-HA-HA-human CMYC (277-439)
Plasmid#203435PurposeMammalian expression of MYCDepositorInsertMYC (MYC Human)
UseTags2 x HAExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-iRFP702
Plasmid#45455DepositorInsertiRFP702
UseTagsExpressionBacterialMutationT2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…PromoteraraBADAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT2T3
Plasmid#50594PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT2, gRNA scaffold, TaU3t plus, U6-26p, T3
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-miRFP703
Plasmid#113097PurposeGeneral donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-miRFP703
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-YPet-WPRE
Plasmid#193342PurposeAAV-mediated expression of YPet under the TRE promoter in mammalian cellsDepositorInsertYPet
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mAmetrine1.1-WPRE
Plasmid#193340PurposeAAV-mediated expression of mAmetrine1.1 under the TRE promoter in mammalian cellsDepositorInsertmAmetrine1.1
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-KLC2-N-18
Plasmid#62759PurposeLocalization: Kinesin Light Chain , N1 Cloning Vector, Excitation:487, Emission:509DepositorInsertKLC2
UseTagsmEmeraldExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPH6
Plasmid#40315DepositorInsertPeptide:N-glycosidase F
UseTagsHexaHistidine and OmpA3 leaderExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbE8c-RFP
Plasmid#35269DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX5 (pcDNA3.1+)
Plasmid#75214PurposeMammalian expression of SLC24A5DepositorInsertSLC24A5 (SLC24A5 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFBP_promoter_acceptor
Plasmid#189920PurposeBinary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1513
Plasmid#143513PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNKX3-1 (NKX3-1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCAT
Plasmid#140084PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_DNAseI
Plasmid#182408PurposeBacterial expression of DNaseIDepositorInsertDNase I (DNASE1 Bovine)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-C1
Plasmid#60560PurposeExpresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteinsDepositorInsertmTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-M
Plasmid#182280PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.DepositorInsertcAMPFIRE-M (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SAR1
Plasmid#67472PurposeMammalian expression of hSar1 with stop codon (native expression)DepositorInsertSAR1 (SAR1A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
retro-puro vector
Plasmid#58250PurposeRetroviral expression vector encoding an empty IRES-Puromycin cassetteDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX552
Plasmid#60958PurposepAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.DepositorInsertsU6_(SpaI)_sgRNA
Syn_EGFP-KASH
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMammalianMutationPromoterSynapsin and U6Available sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
FSW-ALDH2-T2A-EGFP
Plasmid#197036PurposeExpress ALDH2 from hSyn1 promoter, co-expressed with EGFPDepositorInsertALDH2 (ALDH2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCP-M-MLV RT∆RNase H
Plasmid#213749PurposeFor circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cellsDepositorInsertMCP-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAP2K2
Plasmid#23555DepositorInsertMAP2K2 (MAP2K2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
Human SERCA2b (pcDNA3.1+)
Plasmid#75188PurposeMammalian expression of ATP2A2DepositorInsertATP2A2 (ATP2A2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGs-Foxp1
Plasmid#45720DepositorInsertFoxP1 (Foxp1 Mouse)
UseTagsExpressionMutationPromoterCMV / b-actinAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-LDLR-mCherry BlastR
Plasmid#186739PurposeDox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertLDLR (LDLR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiV2-dCas9-VP64
Plasmid#141104PurposeExpression of gRNA and dCas9-VP64 from the same plasmid for CRISPRaDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP1_Z
Plasmid#148150PurposeMammalian Expression of Hs4EBP1DepositorInsertHs4EBP1 (EIF4EBP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only