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Plasmid#111319Purposesnu114(G145A)DepositorInsertSNU114
UseTagsExpressionYeastMutationsnu114 G145APromoterAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1201 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xΦ]
Plasmid#107846PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll hydrophobics combined in six clusters in spli…PromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1138 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆NTD]
Plasmid#107842PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of N-terminal domain in splicing reporterPromoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1109 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆CTD]
Plasmid#107841PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of C-terminal domain in splicing reporterPromoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHBS1108 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆RRM]
Plasmid#107840PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of RRM domains in splicing reporterPromoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
P0050::kaiBC
Plasmid#101828Purposedrives expression of kaiBC from an rpaA-independent promoterDepositorInsertkanamycin casette and p0050 promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::glgP_gap1
Plasmid#101845PurposeReplaces native promoter of glgP_gap1 with Ptrc promoterDepositorInsertCmR casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::zwf_fbp_opcA
Plasmid#101846PurposeReplaces native promoter of zwf_fbp_opcA with Ptrc promoterDepositorInsertsp/st resistance casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::gnd
Plasmid#101844Purposedrives expression of gnd using Ptrc promoterDepositorInsertKan casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
zwf-fbp-opcA-
Plasmid#101839Purposedeletes zwf_fbp_opcA locusDepositorInsertKanamycin casette (in place of synpcc7942_2333-2335 operon)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_EF_syn_BbsI
Plasmid#89921PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSE and FSF (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_HI_syn_BbsI
Plasmid#89924PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSH and FSI (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB1_T_34_ani_trpC
Plasmid#90291PurposeBB1 terminator trpC gene Aspergillus nidulans between FS3 and FS4DepositorInserttrpC
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB1_L_12_syn_BsaI
Plasmid#89914PurposeLinker to construct BB1 from PCR product with FS1 and FS2 (in with BsaI, out with BbsI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB1_L_34_syn_BsaI
Plasmid#89916PurposeLinker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_AB_syn_BbsI
Plasmid#89917PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSA and FSB (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_BC_syn_BbsI
Plasmid#89918PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSB and FSC (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_CD_syn_BbsI
Plasmid#89919PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSC and FSD (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_DE_syn_BbsI
Plasmid#89920PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_FG_syn_BbsI
Plasmid#89922PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only