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pcDNA_DiCre_60.F2

Plasmid

#60208

Purpose

Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Human, Synthetic, Bacteriophage P1)

Use

Tags

FRAP and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 2021-2112 of FRAP with a T2098L mutat…

Promoter

CMV

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA-SCW-HHL

Plasmid

#190634

Purpose

Mammalian expression of genetically encoded calcium indicator SCW-HHL

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW-HHL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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psgRNA2.02-MS2-ccdB

Plasmid

#99911

Purpose

Basic cloning vector for the expression of MS2 inserted sgRNA

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Bbs4

Plasmid

#128868

Purpose

For mammalian expression of mouse Bbs4

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Bbs4 (Bbs4 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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VfAU1-LOV_P2A_casp9

Plasmid

#131224

Purpose

Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

VfAU1-LOV-HA_P2A_myc-casp9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Bongwoori

Plasmid

#63720

Purpose

Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D

Depositor

Bradley Baker

Article

Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

Insert

Bongwoori

Use

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

Plasmid

#169591

Purpose

Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP and iRFP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin-2

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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PA-RL-mCherry

Plasmid

#78861

Purpose

DULIP negative control (bait) to exclude unspecific background binding

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

mCherry

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-CAML

Plasmid

#22284

Depositor

Erik Lillehoj

Article

Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6

Insert

calcium-modulating ligand (CAMLG Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2009

Availability

Academic Institutions and Nonprofits only
b2-TS3

Plasmid

#103877

Purpose

full-length human b2 with tension sensor at b2_733

Depositor

Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

full-length human b2 with tension sensor at b2_733 (ITGB2 Human)

Use

Tags

tension sensor module

Expression

Mutation

Promoter

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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deltaPYD-NLRP11.MYC

Plasmid

#131197

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

deltaPYD-NLRP11 (NLRP11 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

deletion of the pyrin domain (amino acids 1-124 m…

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-del.GW-I+II+III-NES-mut

Plasmid

#42040

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP and Myc

Expression

Mammalian

Mutation

deleted aa457-532, aa716-772 and aa804-924, F951A…

Promoter

CMV

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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aL-mEos3.2

Plasmid

#113154

Purpose

constrained LFA-1-mEos3.2 fusion

Depositor

Timothy Springer

Article

Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

Insert

full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)

Use

Tags

mEOS3.2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTS1026-Tier1-PhCMV-3xNLS-iRFP670

Plasmid

#169532

Purpose

Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nucleus-localized far-red fluorescent protein iRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCY2

Plasmid

#59016

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

Insert

NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor

Use

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
CMV-2xFLAG-Atoh1ER-DsRed

Plasmid

#101735

Purpose

Expresses mouse Atoh1 fused to ER and DsRed

Depositor

Albert Edge

Article

Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2x FLAG, DsRed, and ER

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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mcherry Beta 4

Plasmid

#36114

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B4 (Gnb4 Mouse)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

cmv

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
mcherry Beta 3

Plasmid

#36113

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B3 (GNB3 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

cmv

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
V5-WT-short-UBASH3A

Plasmid

#192088

Purpose

Mammalian expression of human wild-type short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2017 Jul;66(7):2033-2043. doi:

Insert

UBASH3A (UBASH3A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPA-RL-FL

Plasmid

#78863

Purpose

DULIP Tandem Construct used for data normalization

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

Firefly

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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