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Plasmid#132258PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertUCP3 (UCP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHEE401E
Plasmid#71287PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 enhancer fused to EC1.1 promoter and U6-26p…Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp16
Plasmid#156482PurposeBacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tagDepositorInsertNsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - EGFP sgRNA 1
Plasmid#51760PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/er-(n2)oxStayGold(c4)v2.0
Plasmid#186296PurposeA new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.DepositorInserter-(n2)oxStayGold(c4)v2.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST
Plasmid#208048PurposeEnables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidinDepositorInsertBirAopt
UseLentiviralTagsExpressionMutationBirAopt is human-codon-optimized BirA (PMID 15707…PromoterEFSAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC013 - Twinstrep-SUMO-huLwCas13a
Plasmid#90097PurposeTwinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.DepositorInsertLwCas13a
UseCRISPRTags6xHis-Twin Strep-SUMOExpressionBacterialMutationPromoterAvailable sinceJune 13, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-opCas12a-6xNLS
Plasmid#209022PurposeLentiviral vector expressing human codon-optimized OpCas12a with 6xNLSDepositorInsertopCas12a-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/S542RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC12A9
Plasmid#131897PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC12A9 (SLC12A9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMXS-mitoGshF-blasticidin
Plasmid#210314PurposeExpresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cellsDepositorInsertBifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
UseRetroviralTagsFLAG peptide, Homo sapiens ACO2 mitochondrial tar…ExpressionMutationCodon optimized for expression in human cellsPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC6A14
Plasmid#131865PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A14 (SLC6A14 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC51A
Plasmid#132240PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC51A (SLC51A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC51B
Plasmid#132157PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC51B (SLC51B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SLC6A19
Plasmid#132231PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC6A19 (SLC6A19 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
EGFP-COV M
Plasmid#165124Purposemammalian expression and localizationDepositorInsertCOV M (M codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SPNS3
Plasmid#132285PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSPNS3 (SPNS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221_SPNS2
Plasmid#132307PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSPNS2 (SPNS2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7Available sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-HF1-NLS-6xHis (gbr2102)
Plasmid#181744Purposebacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY-HF1
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…PromoterT7Available sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHEE401
Plasmid#71286PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertssgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 promoter and U6-26p Arabidopsis U6 gene pro…Available sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only