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Plasmid#96985PurposeExpress yeast SEIPIN gene in plants.DepositorInsertScSEIPIN (SEI1 Budding Yeast)
UseTags6xHis and GFPExpressionPlantMutationN-terminal 39 nucleotides were deleted from yeast…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFAB806
Plasmid#47849PurposeBIOFAB reporter plasmid for measuring lambda tR2 termination efficiencyDepositorInsertlambda tR2 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO4Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB805
Plasmid#47850PurposeBIOFAB reporter plasmid for measuring tonB termination efficiencyDepositorInserttonB terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO5Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB816
Plasmid#47851PurposeBIOFAB reporter plasmid for measuring ilvGEDA termination efficiencyDepositorInsertilvGEDA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO6Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB807
Plasmid#47852PurposeBIOFAB reporter plasmid for measuring RNAI termination efficiencyDepositorInsertRNAI terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO7Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB820
Plasmid#47855PurposeBIOFAB reporter plasmid for measuring tetAC termination efficiencyDepositorInserttetAC terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO10Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB1910
Plasmid#47859PurposeBIOFAB reporter plasmid for measuring rpoC termination efficiencyDepositorInsertrpoC terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO14Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB804
Plasmid#47848PurposeBIOFAB reporter plasmid for measuring T3 early termination efficiencyDepositorInsertT3 early terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO3Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3905
Plasmid#47808PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD1 efficiency.DepositorInsertpromoter 14 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)
Plasmid#139448PurposeLentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoterDepositorInsertFLAG-tag E2-crimson
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl_NHEJ
Plasmid#91249PurposeCRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HRDepositorInsertsCodon optimized dCas9-Mxi1
KU70 sgRNA expression cassette
KU80a sgRNA expression cassette
KU80b sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl
Plasmid#91248PurposeCRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-Mxi1
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-dSaCas9-KRAB-bGHpA
Plasmid#106219PurposeAAV expressing deactivated S. aureus Cas9 fused to a KRAB repressorDepositorInsertdead S. aureus Cas9 KRAB
UseAAV and CRISPRTagsHA-NLSExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-R-GECO1
Plasmid#32444PurposeMammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertR-GECO1.0
UseTagsExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hDDX50-HA
Plasmid#165465PurposeHuman DDX50 mammalian expression vector with C-terminal HA tag.DepositorInsertDExD-box helicase 50 (DDX50 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-PA-Rac1
Plasmid#22027PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseTags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pC034 - LwCas13a-msfGFP-2A-Blast
Plasmid#91924PurposeExpresses active LwCas13a for mammalian RNA targeting with Blasticidin selection markerDepositorInsertLwCas13a
UseCRISPR and LentiviralTagsNLS and msfGFP-NLS-3xHA, P2A, BlasticidinExpressionMammalianMutationPromoterEFSAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC015 - dLwCas13a-NF
Plasmid#91905PurposeExpresses negative-feedback dLwCas13a for mammalian RNA targeting/imagingDepositorInsertdLwCas13a
UseCRISPRTagsNLS and eGFP, ZF CCR5TC, KRAB-A, NLSExpressionMammalianMutationR474A and R1046APromoterCAGAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-R-GECO
Plasmid#32462PurposeMammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorArticleInsertR-GECO1.0
UseTagsNLS sequence DPKKKRKVExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only