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pSiren_EGFP-shHmga1#1

Plasmid

#122288

Purpose

Knockdown for mouse Hmga1

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for mouse Hmga1 (Hmga1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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coreR1 (384-1008)

Plasmid

#115646

Purpose

expression of truncated Rag1

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag1 core (384-1008) (Rag1 Mouse)

Use

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

truncated Rag1 (aa 384-1008)

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch1#1/Cre

Plasmid

#193223

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch1#1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Kir6.2 K+ channel scFv [N363/71]

Plasmid

#190546

Purpose

Mammalian Expression of Kir6.2 K+ channel scFV. Derived from hybridoma N363/71.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj11 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFbxw7#1/Cre

Plasmid

#173635

Purpose

Expresses a Fbxw7-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Fbxw7 (Fbxw7 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFat1#1/Cre

Plasmid

#173633

Purpose

Expresses a Fat1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Fat1 (Fat1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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IDP427

Plasmid

#87021

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

SNAP-Nlg1AB-tdMS2(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pIRES.Puro.mBeta

Plasmid

#24175

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Jan 22. ():.

Insert

pIRES.Puro.mBeta (Polb Mouse)

Use

Tags

Expression

Mammalian

Mutation

x

Promoter

Available since

April 9, 2010

Availability

Academic Institutions and Nonprofits only
pCR4-MBII52-probe

Plasmid

#67647

Purpose

antisense RNA probe for RNase protection or nothern

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII52 snoRNA (Snord115 Mouse)

Use

For in vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
tdEos-Talin-N-22

Plasmid

#57673

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PGEX 4T-1-PPARgamma2

Plasmid

#78773

Purpose

To purify GST-tagged recombinant protein

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_2

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
RAP2A

Plasmid

#55666

Purpose

expression of mouse RAP2A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RAP2A (Rap2a Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
piiTRE-Bi-SG-Tii

Plasmid

#26121

Depositor

Liqun Luo

Article

Li et al PLoS One. 2010 . 5(7):e11503.

Insert

Synaptophysin (Syp Mouse)

Use

Tags

mut4EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only
GAL4-mNFAT1(2-144)

Plasmid

#11108

Purpose

Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

Depositor

Anjana Rao

Article

Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

Insert

NFAT1 (Nfatc2 Mouse)

Use

Tags

GAL4-DBD

Expression

Mammalian

Mutation

Contains NFAT1 amino acids 2-144; L78P and P140Q …

Promoter

Available since

April 24, 2006

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta6

Plasmid

#100883

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 489-781aa

Promoter

CMV

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-NEEP21-N-18

Plasmid

#57407

Purpose

Localization: Endosomes, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

NEEP21

Use

Tags

mEos2

Expression

Mammalian

Mutation

D114G

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pSG5-FLAG-mEKLF

Plasmid

#67833

Purpose

Expression of FL-EKLF driven by SV40 promoter

Depositor

James Bieker

Article

Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

EKLF (Klf1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

SV40

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pCWXPGR-pTF-DVL2deltaDIX

Plasmid

#114276

Purpose

TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

DVL2 (Dvl2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

deletion of DIX domain

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Kir2.1Mut-2A-mScarlet-KASH

Plasmid

#203841

Purpose

non-functional Kir2.1(mutant) expression as a control for the silencing of neurons with Kir2.1 and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Expression

Mutation

G144A, Y145A, G146A

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-Cavin1-GFP

Plasmid

#170859

Purpose

Mammalian expression of His-Cavin1-GFP.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

caveolae associated 1 (Cavin1) (Cavin1 Mouse)

Use

Tags

(His)6 and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRDAX-505 C-Tag GFP[2] L10 Itgb1 EBPF2

Plasmid

#130032

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Itgb1 (Itgb1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBRPyCAG-fmKlf4-DsRed-Ip

Plasmid

#26274

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Klf4 (Klf4 Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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Dlx6

Plasmid

#15539

Depositor

John Rubenstein

Article

Liu et al Dev Dyn. 1997 Dec . 210(4):498-512.

Insert

Dlx6 (Dlx6 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
Dendra2-CAF1-C-10

Plasmid

#57702

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

CAF1

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCEP4-myc-H2-Ld

Plasmid

#135519

Purpose

Mammalian expression of myc-tagged H2-Ld

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Ld

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Nanog-luciferase targeting construct

Plasmid

#26591

Depositor

Rudolf Jaenisch

Article

Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

Insert

Nanog promoter-luciferase-lox-pgk-neomycin-loc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pLV Tmx1-V5

Plasmid

#175138

Purpose

Lentiviral expression of V5-tagged mouse Tmx1

Depositor

Larry Gerace

Insert

Tmx1 (Tmx1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Klf4

Plasmid

#136613

Purpose

Dox-inducible lentiviral vector expressing mouse Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Klf4 (Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCFL-mPML-2CAMut-iB

Plasmid

#140286

Purpose

Expression of mouse PML Ca mutant

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

PML (Pml Mouse)

Use

Tags

Expression

Mammalian

Mutation

CA mutant (RING finger mutant)

Promoter

CAG

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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Myopathy Loop Mutant Nuclear Myosin I YFP

Plasmid

#60243

Purpose

Expresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activity

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

Deleted I339, I340, G343, E344, E345,L346

Promoter

CMV

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only