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Plasmid#23782DepositorInsertPBK (PBK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMy-MouseCTCFbiotag-T2A-mOrange
Plasmid#50564PurposeRetroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-termDepositorInsertsAmpicilin
Mouse CTCF Exons
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP
Plasmid#216475Purposelentiviral dual expression of YAP5SA and eGFPDepositorInsertsYAP5SA
eGFP
UseLentiviralTagsExpressionMutationS61A, S109A, S127A, S164A, S381APromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
phage ubc nls ha 2xmcp HALO
Plasmid#64540PurposeUsed to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.DepositorInsertMCP
UseLentiviralTagsHA, HALO, and NLSExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Padron2
Plasmid#172355PurposeExpresses the reversibly switchable fluorescent protein Padron2 in bacterial cellsDepositorInsertPadron2
UseTags6xHisTag (N terminal on backbone)ExpressionBacterialMutationPromoteraraBADAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSaPIPLC His
Plasmid#173810PurposeExpression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertStaphylococcus aureus plc
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUltra-sY
Plasmid#82417PurposeExpresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codonsDepositorInsertMethanococcus jannaschii aaRS for sulfotyrosine
UseTagsExpressionBacterialMutationTyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…PromotertacIAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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mCyRFP1-Cdc42
Plasmid#84360Purposefusion protein of Cdc42, FRET donorDepositorInsertmCyRFP1-Cdc42
UseTagsmCyRFP1 N terminal fusion to Cdc42ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf6(WT)-HA
Plasmid#79424PurposeExpresses C-terminally HA-tagged Arf6(WT) in mammalian cellsDepositorInsertArf6 (ARF6 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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PB_iETV2_P2A_GFP_Puro
Plasmid#168805PurposePiggyBac transposon construct with doxycycline inducible ETV2 + GFP expressionDepositorInsertETV2 (ETV2 Human, Synthetic)
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKmK.T1
Plasmid#125053PurposeStorage plasmid for K.marxianus terminator; type 4 partDepositorInsertINU1 terminator
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-Cre
Plasmid#182736PurposeCre expression under the control of CamKII promoterDepositorInsertCre
UseAAVTagsExpressionMutationPromoterCamKIIAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-His-tag-hphMX4
Plasmid#20764DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
eNme2C-CBE6b
Plasmid#215830PurposeExpress CBE6b (with eNme2-C variant) in mammalian cellsDepositorInsertCBE6b-eNme2C-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hHNF4alpha
Plasmid#206878PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.DepositorInsertHuman hepatocyte nuclear factor 4 alpha (HNF4A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HIS2-Vps2
Plasmid#21494DepositorInsertVPS2 (DID4 Budding Yeast)
UseTags6x His and MBPExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro human IRS-1
Plasmid#11025DepositorInsertIRS-1 (IRS1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
PCT62
Plasmid#170391Purposeexpresses three sgRNA cassettesDepositorInsertPCT62-targetsite
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-PPARdelta
Plasmid#16529DepositorInsertPPAR delta (PPARD Human)
UseAdenoviralTags2x HA (YDVPDYAS)ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pIREShygro HA-mVenus-mAID
Plasmid#117714PurposeExpression of HA-tagged mVenus fused to mAIDDepositorInsertmVenus-mAID
UseTagstriple HAExpressionMammalianMutationcodon optimized mAID for murine expressionPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct_mScarlet-H_N1
Plasmid#85055PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorInsertLifeAct
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Kv2.1/RBG4
Plasmid#196947PurposePlasmid expressing an antigen targeting Kv2.1 (RBG4)DepositorInsertKv2.1 (Kcnb1 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJB6
Plasmid#177657PurposeRecombinant protein synthesis of human DNAJB6DepositorInsertDNAJB6 (DNAJB6 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCitrine
Plasmid#188386PurposeLentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;DepositorInsertGal4UAS_tBFP_PGK_mCitrine
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Plasmid#32702PurposeConditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.DepositorInserteGFP
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDest-795
Plasmid#161881PurposeStandard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsMBPExpressionInsectMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TGFBR2
Plasmid#116800PurposeLentiviral expression of TGFBR2DepositorInsertTGFBR2 (TGFBR2 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
rnLbCas12a-PE2
Plasmid#213746PurposeFor circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cellsDepositorInsertrnLbCas12a-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R, H759A, R1138A in LbCas12aPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only