We narrowed to 57,915 results for: plasmid
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Plasmid#105534PurposeAAV expression of LacZ from TBG promoterDepositorHas serviceAAV8InsertLacZ
UseAAVExpressionMammalianPromoterTBGAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-fDIO-mCherry-WPREpA
Plasmid#177328PurposeControl vector for Flp-dependent DREADD constructsDepositorInsertmCherry
UseAAV; Flp-frtExpressionMammalianPromoterHuman Synapsin-1Available sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
HO-hisG-URA3-hisG-poly-HO
Plasmid#51663Purposeyeast plasmid for integration of target sequence at HO locus, URA3 selectionDepositorTypeEmpty backboneUseYeast integrationExpressionYeastAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.3_SARS2_XBB.1.5
Plasmid#196585Purposeexpressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus productionDepositorInsertSpike (XBB.1.5)
ExpressionMammalianMutationG252V, F486P on the basis of XBBPromoterCMVAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRAD1
Plasmid#47697PurposeGeneral cloning vector for use in E. coli or D. radioduransDepositorTypeEmpty backboneExpressionBacterialPromoterrepUAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SAR1-GFP
Plasmid#67409PurposeMammalian expression of hSar1 with C-term GFP tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSAR1 (SAR1A Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT/CMV-SV40-LgT
Plasmid#20204DepositorInsertSV40LgT
UseTransposonAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGL3Luc-5XNF-kappaB
Plasmid#185695PurposeReporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNF-kappaB (NFKB1 Human)
UseLuciferasePromoterNF-kappaB binding siteAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cys-3HB-SPYcatcher
Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAd5-B1
Plasmid#122551PurposeBlock 1 for AdenoBuilder genome assemblyDepositorInsertAdenovirus 5 genomic region 1-3759
UseSynthetic BiologyPromoterNoneAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInt-ts
Plasmid#66076PurposeThe pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda versionDepositorInsertlambda integrase
Available sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p-dCas9-HDAC1-Hygro
Plasmid#104409Purposetransient expression of dCas9-HDAC1 fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHDAC1 (HDAC1 Human)
UseCRISPRExpressionMammalianPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS303-PADH1-AFB2
Plasmid#99530PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)DepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-FLEX-RiboL1-jGCaMP8s
Plasmid#192616PurposeAAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.DepositorInsertFLEX-RIboL1-jGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEMS2115
Plasmid#49140PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-emGFP-WPRE
UseAAVExpressionMammalianPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
kiCAP-AAV-MyoAAV4A
Plasmid#224448PurposeRep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRGDYNSL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-28:TUBA1B-mTagRFP-T
Plasmid#101785PurposeHomology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCH45 (multiAsCas12a-KRAB lenti)
Plasmid#217330PurposeLentiviral expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPR and LentiviralTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2MutationR1226A/E174R/S542R/K548RPromoterUCOE-SFFVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgAAVS1
Plasmid#192477PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a controlDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9
TagsT2A iRFP670ExpressionMammalianAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)
Plasmid#185911PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized ABE8e-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by