Addgene

Showing: 3601 - 3620 of 5035 results

Results per page:

Sort results by:

FLAG-ZnF/HIT1

Plasmid

#15332

Depositor

Ronald Conaway, Joan Conaway

Article

Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

Insert

zinc finger, HIT domain containing 1 (ZNHIT1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pGAL4-DBD-MCS-long

Plasmid

#145246

Purpose

Backbone vector expressing GAL4 DNA-binding domain followed by MCS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-jGCaMP8f F366H

Plasmid

#204141

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Use

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Phenylalanine 366 changed to histidine

Promoter

CMV

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Flag1-3HABeta2-mEos2

Plasmid

#114187

Purpose

studying clustering of Beta2 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Article

Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

Insert

Adrenergic receptor beta 2 (ADRB2 Human)

Use

Tags

3HA, FLAG, and mEOS2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Dr-EGFR

Plasmid

#184004

Purpose

Dr-EGFR expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

Insert

Dr-EGFR (EGFR Human)

Use

Tags

Deinococcus radiodurans bacterial phytochrome

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-CD28TMD-NTEVp(H75S)

Plasmid

#137828

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(H75S)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Histidine 75 to Serine in NTEVp fragment

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EcPPK1

Plasmid

#108850

Purpose

Expresses E. coli PPK1 in mammalian cell lines

Depositor

Michael Downey

Article

Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

Insert

Polyphosphate Kinase 1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

Plasmid

#188993

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Use

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

D201G, A258T, K327R, G328S, G381V, G429D, T450A

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-ERK2 L198A L232A

Plasmid

#8978

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

L198A, L232A

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
GFP-Golgin-84-TEV #709#243

Plasmid

#42108

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

Golgin-84 (GOLGA5 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

inserted ENLYFQG b/w 300 and 301

Promoter

CMV

Available since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
pSSA-PMG (pXK-1649)

Plasmid

#208857

Purpose

SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

Depositor

Kun Xu

Article

Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-CD28TMD-NTEVp(WT)

Plasmid

#137826

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(WT)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 PBD wt (Nigg AH12)

Plasmid

#41162

Depositor

Erich Nigg

Article

Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

Insert

PBD (PLK1 Human)

Use

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Contains Plk1-PBD only (aa 326–603)

Promoter

CMV

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pER-eZinCh-2

Plasmid

#70009

Purpose

expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

Depositor

Maarten Merkx

Article

Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

Insert

ER-eZinCh-2

Use

Tags

KDEL sequence and PPI signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dMASS_1

Plasmid

#209764

Purpose

Expression of dMASS_1 in mammalian cells under CMV promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-NES-ZapCmR2

Plasmid

#59015

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor

Use

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
mGFAP-Halo

Plasmid

#169474

Purpose

the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

GFAP (Gfap Mouse)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Changed R 236 to H

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Opto-M2R

Plasmid

#106070

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M2R; Opto-M2R; F12; 723)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-M2R

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
psgRNA2.02-PP7-ccdB

Plasmid

#99912

Purpose

Basic cloning vector for the expression of PP7 inserted sgRNA

Depositor

Yujie Sun

Article

Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-YL1

Plasmid

#15333

Depositor

Ronald Conaway, Joan Conaway

Article

Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

Insert

transcription factor-like 1 (VPS72 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only