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Flag-hPKL WT

Plasmid

#107159

Purpose

For mammalian expression of Flag-hPKL WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL WT (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLX303-PCDH7-a

Plasmid

#108538

Purpose

Expresses human PCDH7 transcript variant a in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant a (PCDH7 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 W201E

Plasmid

#186477

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1REST_sh_seq2_WPRE

Plasmid

#127574

Purpose

shRNA to knock down RE1-silencing transcription factor (REST)

Depositor

Malin Parmar

Article

Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

Insert

shRNA for knock down of REST (REST Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLX303-PCDH7-d

Plasmid

#108541

Purpose

Expresses human PCDH7 transcript variant d in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant d (PCDH7 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGex-4T-2_LC3A

Plasmid

#73946

Purpose

For recombinant expression of human LC3A/MAP1LC3A in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

LC3A (MAP1LC3A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
BAF-WT-tet-puro

Plasmid

#199648

Purpose

Doxycycline-inducible BAF expression

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

BAF (BANF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pNK2657

Plasmid

#219741

Purpose

MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

hispidin- synthase from Mycena citricolor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-REST_sh_seq1_WPRE

Plasmid

#127573

Purpose

shRNA to knock down RE1-silencing transcription factor (REST)

Depositor

Malin Parmar

Article

Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

Insert

shRNA for knock down of REST (REST Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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GLE1

Plasmid

#155625

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

GLE1 (GLE1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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FOR human OCT4 TALEN

Plasmid

#52412

Purpose

TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

HD NG NN NN NN HD NG HD NG HD HD HD NI NG

Use

TALEN

Tags

Expression

Mutation

Promoter

CAGGS

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only
R26-dCas9-VPR-mNG

Plasmid

#172441

Purpose

Rosa26 targeting vector, expresses dCas9-VPR and mNeonGreen

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

dCas9-VPR
mNeonGreen

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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THOC4

Plasmid

#156083

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC4 (ALYREF Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLuc-WPRE-HGHpA

Plasmid

#87951

Purpose

Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

Depositor

Mark Kay

Article

Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

Insert

Firefly Luciferase

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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U6-sgfiller-eCas9-T2A-BlastR

Plasmid

#172437

Purpose

Expresses eCas9, BlastR and sgRNA cloning site

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

eCas9
BlastR

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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DHX58

Plasmid

#155542

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DHX58 (DHX58 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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BAF-S4A-tet-puro

Plasmid

#199649

Purpose

Doxycycline-inducible BAF expression

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

BAF (BANF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S4A

Promoter

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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BAF-S4D-tet-puro

Plasmid

#199650

Purpose

Doxycycline-inducible BAF expression

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

BAF (BANF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

S4D

Promoter

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMG059 MBP-CREB-His

Plasmid

#154076

Purpose

Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-LRRK2 Armadillo 1-159

Plasmid

#186016

Purpose

2xHis-LRRK2 Armadillo 1-159

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)

Use

Tags

Expression

Bacterial

Mutation

See depositor's comments below.

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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