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Plasmid#221693PurposeMammalian expression of FLAG-tagged human p31(comet) (Q83A/F191A)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationQ83A, F191APromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-Flag-RECQL5
Plasmid#222620PurposeMammalian expression vector of flag-tagged RECQL5DepositorInsertRECQL5 (RECQL5 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-Flag-BRIP1
Plasmid#222619PurposeMammalian expression vector of flag-tagged BRIP1DepositorInsertBRIP1 (BRIP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJL1-sFLAG-p5-PRB4_R7-9-FETCH1
Plasmid#210651PurposeIn vitro expression of polyProline FETCH reporter from the T7 promoterDepositorInsertsFLAG-p5-PRB4_R7-9-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sFLAG-p1-PRB4_R7-9-FETCH1
Plasmid#210652PurposeIn vitro expression of polyProline FETCH reporter from the T7 promoterDepositorInsertsFLAG-p1-PRB4_R7-9-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sFLAG-a5-PRB4_R7-9A-FETCH1
Plasmid#210653PurposeIn vitro expression of polyProline FETCH control from the T7 promoterDepositorInsertsFLAG-a5-PRB4_R7-9A-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FP5369 KIF17MD-flag- FIREmate
Plasmid#216724PurposeExpresses motor domain of KIF17 fused to FIREmate in mamallian cellsDepositorInsertKIF17 (KIF17 Human)
UseTagsFIREmate and FlagExpressionMammalianMutation1-547PromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-3xFLAG-NFATC2IP
Plasmid#221208PurposeMammalian expression vector of triple Flag tagged NFATC2IPDepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlagExpressionMammalianMutation1st Methionine deletedPromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-3xFLAG-TAF5-∆ex8
Plasmid#209050PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8)DepositorInsertTAF5 deltaexon-8
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-TAF5-FL_WobbleMut
Plasmid#209055PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform mutated to be resistant to TAF5 siRNADepositorInsertTAF5 full length
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-TAF5-∆ex8_WobbleMut
Plasmid#209056PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNADepositorInsertTAF5 deltaexon-8
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt1
Plasmid#220244PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 1 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR27A, R31A, F165APromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-TEX264-SIM1_mut-cFlag
Plasmid#220205PurposeMammalian expression of TEX264 with SIM1 motif mutated, C-terminal FlagDepositorInsertTEX264 (TEX264 Human)
UseTagsFlagExpressionMammalianMutationp.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…PromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-TEX264-SIM2_mut-cFlag
Plasmid#220206PurposeMammalian expression of TEX264 with SIM2 motif mutated, C-terminal FlagDepositorInsertTEX264 (TEX264 Human)
UseTagsFlagExpressionMammalianMutationp.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…PromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-TEX264-E194A-cFlag
Plasmid#220207PurposeMammalian expression of TEX264 with E194A mutation, C-terminal FlagDepositorInsertTEX264 (TEX264 Human)
UseTagsFlagExpressionMammalianMutation194E>APromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-TEX264-SHP1mut-cFlag
Plasmid#220208PurposeMammalian expression of TEX264 with SHP domain mutated, C-terminal FlagDepositorInsertTEX264 (TEX264 Human)
UseTagsFlagExpressionMammalianMutationp.280GEGPL>RERPA; c.838G>C,844G>A,850T&g…PromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-APEX3-C Cloning Vector
Plasmid#220615PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)DepositorTypeEmpty backboneUseTagsFlag-APEX3 fusionExpressionMammalianMutationPromoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
STREP-SUMO-FLAG-SeKaiC
Plasmid#218179PurposeWT SeKaiC for phosphorylation/dephosphorylation studyDepositorInsertSTREP-SUMO-FLAG-SeKaiC
UseTagsSTREP-TagExpressionBacterialMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-Flag-Miwi-EGFP
Plasmid#212575PurposeLentiviral expression of Flag-tagged mouse Miwi in mammalian cellsDepositorInsertMiwi (Piwil1 Mouse)
UseLentiviralTags5x Flag and SNAP tagExpressionMammalianMutationPromoterSFFVAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA1-FLAG
Plasmid#217678PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRA1 (ADGRA1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only