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pENTR-GFP-Patronin CC+CKK

Plasmid

#59048

Purpose

for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Use

Tags

eGFP

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 534–1457

Plasmid

#59050

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Use

Tags

eGFP

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179

Plasmid

#91912

Purpose

Expresses LbnCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbnCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC021 - LbmCas13a from Lachnospiraceae bacterium MA2020

Plasmid

#91911

Purpose

Expresses LbmCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbmCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC026 - PpCas13a from Paludibacter propionicigenes WB4

Plasmid

#91916

Purpose

Expresses PpCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

PpCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847

Plasmid

#91914

Purpose

Expresses CgCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

CgCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-Patronin CC 638–1457

Plasmid

#59051

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Use

Tags

eGFP

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 776–1457

Plasmid

#59052

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Use

Tags

eGFP

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pC025 - Cg2Cas13a from Carnobacterium gallinarum DSM 4847

Plasmid

#91915

Purpose

Expresses Cg2Cas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

Cg2Cas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGB1-mCRT(P-domain)

Plasmid

#83519

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Use

Tags

GB1 and His

Expression

Bacterial

Mutation

P domain (contains residues 187-283)

Promoter

T7

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pGB1-mCRT(C-domain)

Plasmid

#83518

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Use

Tags

GB1 and His

Expression

Bacterial

Mutation

C domain (contains residues 340-399)

Promoter

T7

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pFAB3906

Plasmid

#47809

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD2 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-BFP-P2A-otPKR-ML

Plasmid

#214365

Purpose

Expression of the Blue Fluorescent Protein - P2A - medium length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

BFP-P2A-otPKRML

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-BFP-P2A-otPKR-SL

Plasmid

#214366

Purpose

Expression of the Blue Fluorescent Protein - P2A - short length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

BFP-P2A-otPKRSL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-BFP-P2A-otPKR-FL

Plasmid

#214364

Purpose

Expression of the Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

BFP-P2A-otPKRFL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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IBMc397

Plasmid

#161955

Purpose

Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc180

Plasmid

#161942

Purpose

Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc226

Plasmid

#161944

Purpose

Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2VF/PA-VSV

Plasmid

#139351

Purpose

Expression of the double mutant of TYK2-V678F-P1104A

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

V362F-V678F-P1104A

Promoter

CMV

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab20

Plasmid

#109609

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab20

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab20 (rab20 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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