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pDmHsp70b

Plasmid

#165785

Purpose

Drosophila HSP70B promoter fragment, shows basal expression that can be further enhanced via heatshock

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Hsp70b

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPERpuro-BglII-Hs4E-T_AC

Plasmid

#148445

Purpose

Mammalian Expression of Hs4E-T-shRNA

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

Hs4E-T-shRNA (EIF4ENIF1 Human)

Expression

Mammalian

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDH013

Plasmid

#157638

Purpose

sMHT-113 fused to GFP and RFP

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH003

Plasmid

#157640

Purpose

sMHT-113 fused to SYNZIP17/18

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17
SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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p3XP3-WhiteA2

Plasmid

#165875

Purpose

Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniwhiteA1

Plasmid

#165872

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha1, lacks attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-C138A

Plasmid

#133911

Purpose

Bacterial expression of His6-Ubc9-C138A

Depositor

Jeffrey Bode

Publication

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 138 to Alanine

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

Plasmid

#132686

Purpose

AAV plasmid encoding mouse ACY1 with C-terminal flag tag

Depositor

Jonathan Long

Publication

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Acylase I (Acy1, Acy1 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG

Plasmid

#132684

Purpose

AAV plasmid encoding mouse PM20D2 with C-terminal flag tag

Depositor

Jonathan Long

Publication

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 2 (Pm20d2 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.CNDP1-flag.WPRE.rBG

Plasmid

#132683

Purpose

AAV plasmid encoding mouse CNDP1 with C-terminal flag tag

Depositor

Jonathan Long

Publication

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Carnosine dipeptidase 1 (Cndp1 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E3E4 (630–1244)

Plasmid

#132628

Purpose

expresses human LRP6 E3E4 (630–1244) in Hi5 insect cell

Depositor

Wenqing Xu

Publication

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (630–1244)

Expression

Insect

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E1E4 (20–1244)

Plasmid

#132629

Purpose

expresses human LRP6 E1E4 (20–1244) in Hi5 insect cell

Depositor

Wenqing Xu

Publication

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (20–1244)

Expression

Insect

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pNLRP11minor.MYC

Plasmid

#131195

Purpose

Mammalian expression of human NLRP11 188A

Depositor

Thomas Kufer

Publication

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188A (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188A

Promoter

pCMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI121

Plasmid

#126061

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP1-10

Use

Synthetic Biology

Tags

Linker (GTGGGGS)

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI122

Plasmid

#126062

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

ccdB Cassette

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-fugu SLC2A4 promoter

Plasmid

#65101

Purpose

Luciferase reporter construct under the fugu (Takifugu rubripes) SLC2A4 promoter.

Depositor

Josep Planas

Publication

Marin-Juez et al PLoS One. 2013 Nov 18;8(11):e80628. doi:

Insert

SLC2A4 promoter

Use

Luciferase

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR sgFTCD_1

Plasmid

#102312

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Publication

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_1 (FTCD Human)

Use

Lentiviral

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgFTCD_2

Plasmid

#102313

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Publication

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_2 (FTCD Human)

Use

Lentiviral

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgHAL

Plasmid

#102315

Purpose

genetic depletion of HAL

Depositor

David Sabatini

Publication

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgHAL (HAL Human)

Use

Lentiviral

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3-mCherry-LKB1(S431A)

Plasmid

#84641

Purpose

mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site

Depositor

Jin Zhang

Publication

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(S431A) (STK11 Synthetic, Human)

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

PKA phosphorylation site Serine 428 mutated to Al…

Promoter

CMV

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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