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pEG BacMam C term 3C eGFP myc

Plasmid

#160685

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 eGFP 3C

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1mut-PA-mCherry

Plasmid

#138251

Purpose

Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

Depositor

Jin Zhang

Publication

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CBX1mut-PAmCherry

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNCST-AvicFP1

Plasmid

#129504

Purpose

Expresses AvicFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AausGFP

Plasmid

#129503

Purpose

Expresses AausGFP constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP4

Plasmid

#129502

Purpose

Expresses AausFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP4

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_004

Plasmid

#123785

Purpose

Encodes mAzamiGreen as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mAzamiGreen

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_036

Plasmid

#123750

Purpose

Encodes mScarlet as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_008

Plasmid

#123703

Purpose

Encodes human pUbc as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

phUbC

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_004

Plasmid

#123699

Purpose

Encodes mouse pPGK1 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pPGK1

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTW072M

Plasmid

#115960

Purpose

Destination Vector

Depositor

Ron Weiss

Publication

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl2 (nsP2 Q739L)

Use

Synthetic Biology

Mutation

nsP2 Q739L

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFA0055

Plasmid

#131774

Purpose

Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

Depositor

Frank Albert

Publication

Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

Insert

20mer CASS5a guide (gCASS5a) and 5' sgRNA

Expression

Bacterial and Yeast

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGEX-4T-1 UBXN2A

Plasmid

#113505

Purpose

Bacterial expression of GST-tagged UBXN2A

Depositor

Hemmo Meyer

Publication

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

UBXN2A (UBXN2A Human)

Tags

GST-Thrombin cleavage site

Expression

Bacterial

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEN6-Ubc5 Sc

Plasmid

#83234

Purpose

pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

Depositor

Gali Prag

Publication

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Inserts

Ubiqutin (UBC Human)
Ubc 5 (UBC5 Budding Yeast)
Uba 1

Tags

His

Expression

Bacterial

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCas_5+7_gfp

Plasmid

#60595

Purpose

encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

Depositor

Christopher Voigt

Publication

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_5+7_gfp

Expression

Bacterial

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCDF1-GRM3 (E767K)

Plasmid

#31801

Depositor

Yardena Samuels

Publication

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed glutamic acid 767 to lysine

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-GRM3 (G561E)

Plasmid

#31799

Depositor

Yardena Samuels

Publication

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed glycine 561 to glutamic acid

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pNEXdelta-EGFP-ApAF

Plasmid

#13335

Depositor

Bong-Kiun Kaang

Publication

Lee et al J Cell Biol. 2006 Sep 11. 174(6):827-38.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Aplysia activating factor

Tags

EGFP

Available since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
pBBR-sfGFP N149TAG-MjtRNA

Plasmid

#242001

Purpose

Expresses MjtRNA and contains sfGFP gene with amber codon at 149 position.

Depositor

Wei Niu

Publication

He et al ACS Synth Biol. 2022 Nov 18;11(11):3724-

Insert

sfGFPN149TAG

Expression

Bacterial

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#228558

Purpose

Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Publication

Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

Insert

dhfCas13d (N2V8)

Use

Lentiviral

Tags

NLS-HA

Mutation

R239A/H244A/R858A/H863A

Available since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB514-35S:TjFLS2-HA

Plasmid

#226413

Purpose

Expressing Trachelospermum jasminoides FLS2 protein in plants

Depositor

Gitta Coaker

Publication

Li et al Nat Plants. 2025 Aug;11(8):1628-1641. do

Insert

TjFLS2

Tags

HA

Expression

Plant

Promoter

35S

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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