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pCIneo-V5-SBP-HseIF4AII_AC

Plasmid

#148439

Purpose

Mammalian Expression of HseIF4AII

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HseIF4AII (EIF4A2 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEPYCdKN0001

Plasmid

#196148

Purpose

Gateway destination vector for protein expression of 9xHis_AtTGA1_HiBit

Depositor

Nicola Patron

Publication

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

9xHis_AtTGA1_HiBit

Expression

Bacterial

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

Plasmid

#188704

Purpose

Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

Depositor

David Tobin

Publication

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNAs

Use

Lentiviral

Expression

Mammalian

Promoter

hUbC

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-DCX-TurboID-V5

Plasmid

#190740

Purpose

Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Publication

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

doublecortin (DCX Human)

Tags

TurboID and V5

Expression

Mammalian

Promoter

CMV

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAB170

Plasmid

#101669

Purpose

Opto-T7RNAP*(69), half expr.

Depositor

Mustafa Khammash

Publication

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH031_proD_LbCas12a-gRNA-GFP_CAM

Plasmid

#183075

Purpose

Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria.

Depositor

Jennifer Doudna

Publication

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA targeting GFP

Use

CRISPR

Expression

Bacterial

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR-Kras

Plasmid

#84637

Purpose

Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted

Depositor

Jin Zhang

Publication

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras

Tags

6xHis, C-terminal targeting sequence from Kras, C…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p5xUAS

Plasmid

#165891

Purpose

Synthetic 5xUAS GAL4 driver response element in enhancer grammar, requires minimal promoter for assembly

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xUAS

Expression

Insect

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcB-His-CD)

Plasmid

#176194

Purpose

expresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operon

Depositor

Mark Saper

Publication

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcBCD

Tags

GfcB-6xHis

Expression

Bacterial

Promoter

Ptac

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBH31(gfcD)

Plasmid

#176199

Purpose

Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

Depositor

Mark Saper

Publication

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

pelC leader-6xHis

Expression

Bacterial

Promoter

T7

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBH31(gfcD-10xHis)

Plasmid

#176200

Purpose

Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

Depositor

Mark Saper

Publication

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Tags

pelC leader-10xHis

Expression

Bacterial

Promoter

T7

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSA10(gfcBCD)

Plasmid

#176193

Purpose

expresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operon

Depositor

Mark Saper

Publication

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcBCD

Expression

Bacterial

Promoter

Ptac

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERKsubDD-Venus-NES

Plasmid

#84630

Purpose

Encodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Publication

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERKsubDD-Venus-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p5xQUAS

Plasmid

#165818

Purpose

Synthetic 5xQUAS QF driver response element in enhancer grammar, requires minimal promoter for assembly

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xQUAS

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDmHsp70b

Plasmid

#165785

Purpose

Drosophila HSP70B promoter fragment, shows basal expression that can be further enhanced via heatshock

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Hsp70b

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPERpuro-BglII-Hs4E-T_AC

Plasmid

#148445

Purpose

Mammalian Expression of Hs4E-T-shRNA

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

Hs4E-T-shRNA (EIF4ENIF1 Human)

Expression

Mammalian

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDH013

Plasmid

#157638

Purpose

sMHT-113 fused to GFP and RFP

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH003

Plasmid

#157640

Purpose

sMHT-113 fused to SYNZIP17/18

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17
SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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p3XP3-WhiteA2

Plasmid

#165875

Purpose

Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMiniwhiteA1

Plasmid

#165872

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha1, lacks attB site

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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