-
Plasmid#205211PurposeThis vector encodes of the synCAM tether (iCAM1) and GFP nanobody LAG43DepositorInsertsynCAM iCAM1 tether and Lag43 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag29-iCAM-1
Plasmid#205208PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG29DepositorInsertsynCAM iCAM1 and Lag29 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag43-iCAM-1
Plasmid#205206PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG43DepositorInsertsynCAM iCAM1 and Lag43 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PIP4K2A-Kina
Plasmid#202745Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorInsertmCherry:FKBP1A(3-108) : [GGSA]4GG :PIP4K2A (33-406) (PIP4K2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-DQNAT
Plasmid#198215PurposepJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tagDepositorInsertMBP_DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-PD-DQNAT
Plasmid#198216PurposepJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tagDepositorInsertPD-DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-AQNAT
Plasmid#198211PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_AQNAT
UseTags6x His Tag and AQNAT mutated (non-permissible) gl…ExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-MOOR
Plasmid#198212PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tagDepositorInsertsfGFP_MOOR
UseTags6x His Tag and MOOR glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYUBcLIC-GFP
Plasmid#183509PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.DepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
TBPL1 KO 1
Plasmid#200210PurposegRNA for TBPL1 KODepositorInsertTBP1 KO 1 (Tbpl1 Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
TBPL1 KO 2
Plasmid#200211PurposegRNA for TBPL1 KODepositorInsertTBP1 KO 2 (Tbpl1 Mouse)
UseTagsExpressionMammalianMutationNonePromoterU6Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot2-W556E-dsRNAres_U
Plasmid#147717PurposeInsect Expression of DmNot2-W556E-dsRNAresDepositorInsertDmNot2-W556E-dsRNAres (Rga Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot2-W556E-dsRNAres_U
Plasmid#147702PurposeInsect Expression of DmNot2-W556E-dsRNAresDepositorInsertDmNot2-W556E-dsRNAres (Rga Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot2-del409-432_T
Plasmid#147625PurposeMammalian Expression of DmNot2-del409-432DepositorInsertDmNot2-del409-432 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-del344-380_T
Plasmid#147629PurposeMammalian Expression of DmNot2-del344-380DepositorInsertDmNot2-del344-380 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-del380-404_T
Plasmid#147630PurposeMammalian Expression of DmNot2-del380-404DepositorInsertDmNot2-del380-404 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-del409-432_T
Plasmid#147631PurposeMammalian Expression of DmNot2-del409-432DepositorInsertDmNot2-del409-432 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot2-del344-380_T
Plasmid#147623PurposeMammalian Expression of DmNot2-del344-380DepositorInsertDmNot2-del344-380 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot2-del380-404_T
Plasmid#147624PurposeMammalian Expression of DmNot2-del380-404DepositorInsertDmNot2-del380-404 (Rga Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot3-F672AY676AF727A_S
Plasmid#147505PurposeMammalian Expression of HsNot3-F672AY676AF727ADepositorInsertHsNot3-F672AY676AF727A (CNOT3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot6-L34EI36E_S
Plasmid#147508PurposeMammalian Expression of HsNot6-L34EI36EDepositorInsertHsNot6-L34EI36E (CNOT6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot3_1-241_K
Plasmid#146795PurposeMammalian Expression of HsNot3_1-241DepositorInsertHsNot3_1-241 (CNOT3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot3_589-753_K
Plasmid#146797PurposeMammalian Expression of HsNot3_589-753DepositorInsertHsNot3_589-753 (CNOT3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsDCP1b_H
Plasmid#146505PurposeMammalian Expression of HsDCP1bDepositorInsertHsDCP1b (DCP1B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsDCP2_G
Plasmid#146401PurposeMammalian Expression of HsDCP2DepositorInsertHsDCP2 (DCP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 3xSA
Plasmid#195207PurposeMammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)DepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationS162/339/340APromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 3xSD
Plasmid#195208PurposeMammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)DepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationS162/339/340DPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 PDZ1/2*
Plasmid#195211PurposeMammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutantDepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationDual PDZ1/2 GYGF>GYAA motif mutantPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I16 tUBQ1 GG5-GG6
Plasmid#195898PurposeLevel 0 tUBQ1 terminator with GG5 GG6 golden gate linkersDepositorInserttUBQ1 (UBQ1 Mustard Weed)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I15 tUBQ1 GG4-GG6
Plasmid#195897PurposeLevel 0 tUBQ1 terminator with GG4 GG6 golden gate linkersDepositorInserttUBQ1 (UBQ1 Mustard Weed)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-miRFP-FRBx2
Plasmid#193569PurposeExpresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells.DepositorInsertPH domain from PLC delta1 (Plcd1 Rat)
UseTagsFRB (T2098L) and miRFP703ExpressionMammalianMutationPH domainPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-mCherry-FRBx2
Plasmid#193570PurposeExpresses PH domain from PLC delta1 tagged with mCherry and two FRB mutants (T2098L) in mammalian cells.DepositorInsertPH domain from PLC delta1 (Plcd1 Rat)
UseTagsFRB (T2098L) and mCherryExpressionMammalianMutationPH domainPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLRX-roGFP2 in pT3TS-Dest
Plasmid#194297PurposeIn vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRKDepositorInsertGLRX-roGFP2
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-p3E-GLRX-roGFP2
Plasmid#194288PurposeMultisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQFDepositorInsertGLRX-roGFP2
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYI006
Plasmid#170840PurposeFRO6p::GUSPlus::tOCS in pFP100DepositorInsertFRO6p::GUSPlus::tOCS
UseTagsExpressionPlantMutationPromoterFRO6Available sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYI046
Plasmid#170842Purpose(pUBQ10::SMT2-FLAG::tOCS)DepositorInsertpUBQ10::SMT2-FLAG::tOCS
UseTagsExpressionPlantMutationPromoterpUBQ10Available sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA
Plasmid#186411PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfA
Plasmid#186408PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP
Plasmid#186412PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-ECFP
Plasmid#186403PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-ECFP
Plasmid#186402PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::RfA-venus
Plasmid#186401PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::venus-RfA
Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-doraWT_rescue_construct
Plasmid#190608PurposePuromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.dora_1
Plasmid#190700PurposeExpresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertdora (CG34401) sgRNA 1
UseCRISPRTagsExpressionInsectMutationPromoterDrosophila U6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.dora_2
Plasmid#190701PurposeExpresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertdora (CG34401) sgRNA 2
UseCRISPRTagsExpressionInsectMutationPromoterDrosophila U6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.dora_3
Plasmid#190702PurposeExpresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertdora (CG34401) sgRNA 3
UseCRISPRTagsExpressionInsectMutationPromoterDrosophila U6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-NLSlacZ-stop-L2
Plasmid#186358PurposeEntry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ E. coli, Synthetic)
UseExpression of nuclear lacz reporterTagsNuclear localization signalExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only