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Plasmid#128337PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorInsertChrna2 (Chrna2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrna4-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128339PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorInsertChrna4 (Chrna4 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1330 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]
Plasmid#107839PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationWildtype in splicing reporterPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBac(3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry)
Plasmid#119064PurposeCre/lox flip-out construct for marking cell clones in Tribolium castaneumDepositorInsertspBacL
pUb regulatory sequence
loxN
mYFP
loxN
H2B-mcherry
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128344PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorInsertChrnb3 (Chrnb3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW811_pCAG-PV8-F14-GFP-T2A-mCherry-BGHpA
Plasmid#87587PurposePV8 for 3-input, 2-output Full AdderDepositorInsertEGFP, mCherry
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49219PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2
Plasmid#231207PurposeSingle transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)DepositorInsertPuro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
UseLentiviralTagsExpressionMutationL147I, R148H, I203V, L290VPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP
Plasmid#223208PurposeLentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertPDL1 (CD274 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-IL35-IRES-mCherry-PGK-BFP
Plasmid#223210PurposeLentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertIL35 (IL12A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-CMV-GFP-P2A-K20-P2A-mCherry
Plasmid#185618PurposeRibosomal stalling reporter: positive controlDepositorInsertGFP-K20(AAA)-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-CMV-GFP-P2A-I15-P2A-mCherry
Plasmid#185620PurposeRibosomal stalling reporter: 15x isoleucineDepositorInsertGFP-I15-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry
Plasmid#226104PurposeStability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cellsDepositorInsertCOX8A (COX8A Human)
UseTagsGFPExpressionMammalianMutationencodes only for mitochondrial targeting sequence…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcs2-cDELE1(142-515)-GFP-IRES-mCherry
Plasmid#226102PurposecDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) stability reporter construct for transient expression in mammalian cellsDepositorInsertDELE1 (DELE1 Human)
UseTagsGFPExpressionMammalianMutationonly encodes for residues 142-515 of DELE1 corres…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Luciferase-P2A-H2A-mCherry (JDW 926)
Plasmid#224488PurposeGateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)DepositorInsertLuciferase-P2A-H2A-mCherry
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)
Plasmid#224532PurposeA Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyADepositorInsertIRES-H2A-mCherry-SV40pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221076PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertGGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221077PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertCCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221079PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertTTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221075PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only