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Plasmid#222720PurposeExpression HIS-FLAG-AtUBQ11 in in bacterial cellsDepositorInsertAtUBQ11 (UBQ11 Mustard Weed)
UseTags2xFLAG and 6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-FLAG-human cGASdeltaN-HA
Plasmid#186899PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Synthetic, Human)
UseRetroviralTagsFLAG and HAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-FLAG-human cGASdeltaN-HA
Plasmid#186848PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Synthetic, Human)
UseRetroviralTagsFLAG and HAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-FLAG-human cGAS-HA
Plasmid#186849PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS with N-terminal FLAG and C-terminal HA tag (Adamts1 Human)
UseRetroviralTagsFLAG and HAExpressionMutationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-Cterm-C-FLAG
Plasmid#222685Purposeoverexpressing C-terminus truncation of TSC22D2 with C-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTSC22D2 C-terminus truncationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-NRBP1-Nterm-N-FLAG
Plasmid#222676Purposeoverexpressing N-terminus truncation of NRBP1 with N-terminal FLAGDepositorInsertNRBP1 (NRBP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationNRBP1 N-terminus truncationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-NRBP1-Nterm-C-FLAG
Plasmid#222677Purposeoverexpressing N-terminus truncation of NRBP1 with C-terminal FLAGDepositorInsertNRBP1 (NRBP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationNRBP1 N-terminus truncationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-NRBP1-Cterm-N-FLAG
Plasmid#222678Purposeoverexpressing C-terminus truncation of NRBP1 with N-terminal FLAGDepositorInsertNRBP1 (NRBP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationNRBP1 C-terminus truncationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-Nterm-N-FLAG
Plasmid#222682Purposeoverexpressing N-terminus truncation of TSC22D2 with N-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTSC22D2 N-terminus truncationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-Nterm-C-FLAG
Plasmid#222683Purposeoverexpressing N-terminus truncation of TSC22D2 with C-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTSC22D2 N-terminus truncationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-Cterm-N-FLAG
Plasmid#222684Purposeoverexpressing C-terminus truncation of TSC22D2 with N-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTSC22D2 C-terminus truncationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLYS6-METTL17-CtoS-FLAG
Plasmid#220876PurposeExpressing METTL17-FLAG_Cys to Ser mutantsDepositorInsertMETTL17 (METTL17 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged Cys to SerPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLYS6-METTL17-LYRMut-FLAG
Plasmid#220877PurposeExpressing METTL17-LYRMut-FLAGDepositorInsertMETTL17 (METTL17 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationChanged the LYP domain to AAAPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-p37-SHPmut-FLAG
Plasmid#225502PurposeInducible expression of FLAG-tagged mutated p37 proteinDepositorInsertp37 (UBXN2B Human)
UseLentiviral; Gateway destination vector, doxycycli…TagsFLAGExpressionMammalianMutationF215A/E218A/QKL220-222AAAPromoterTRE promoter, Tet ONAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-FLAG-HA-Regnase-3 WT
Plasmid#222651PurposeFor in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseIn vitro transcriptionTagsFLAG and HAExpressionMutationPromoterT7Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI_FLAG-GFP(Y39TAG)-6His
Plasmid#223507PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
UseTags6xHis and FLAGExpressionMammalianMutationY39TAGPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD_FLAG-GFP(Y39TAG)-6His
Plasmid#223510PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
UseTags6xHis and FLAGExpressionBacterialMutationY39TAGPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro 3XFLAG-hATXN7L3
Plasmid#225725PurposeTransfection of ATXN7L3 with 3XFLAG tag in mammalian cellsDepositorInsertATXN7L3 (ATXN7L3 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-FLAG-msMMS22L
Plasmid#221015Purposemammalian expression vector of Flag tagged mouse MMS22LDepositorInsertMMS22L (Mms22l Mouse)
UseTagsFlagExpressionMammalianMutationnaturally siResistant to siMMS22L (human) aagacuu…PromoterCMVAvailable sinceSept. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-FLAG-p31(comet)
Plasmid#221692PurposeMammalian expression of FLAG-tagged human p31(comet)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only