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Plasmid#179514PurposeTunable and temporal expression control of SPI1DepositorInsertSPI1 (SPI1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GS PromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG-IRES2-mTagBFP2
Plasmid#175509PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-Six1-myc
Plasmid#138530PurposeInducible expression of Six1 with a myc tagDepositorInsertSix1 (Six1 Mouse)
UseTags3x myc tagExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG-IRES2-mNeonGreen
Plasmid#175506PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG
Plasmid#129758PurposeDox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseLentiviralTags3xFLAGExpressionMammalianMutationchanged Glutamic acid 36 to Alanine and Leucine 3…PromoterTRE3Gs and Tet inducible promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD NFIL3
Plasmid#179512PurposeTunable and temporal expression control of NFIL3DepositorInsertNFIL3 (NFIL3 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GS PromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
hMLKL-Venus
Plasmid#106078Purposeexpression in mammalian cellsDepositorInserthuman MLKL-Venus (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsVenusExpressionMammalianMutationPromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE
Plasmid#176273PurposeExpression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.DepositorInsertCasMINI sgRNA (sgTet) and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-NES
Plasmid#199545Purposeexpresses LOV-Turbo in the mammalian cytosol, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsNES and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-NLS
Plasmid#199663Purposeexpresses LOV-Turbo in the mammalian nucleus, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsNLS and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-TO-PAX7
Plasmid#204346PurposeKnock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.DepositorInserthuman PAX7
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-ERM
Plasmid#199664Purposeexpresses LOV-Turbo on the mammalian ER membrane, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsSEC61B and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Neo-EF1A>Tet3G
Plasmid#184379PurposeThe Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)DepositorInsertTet3G
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro Xlone SOX17
Plasmid#179838PurposeDoxycycline-inducible expression of human SOX17DepositorInsertSOX17 (SOX17 Human)
UseAAV, CRISPR, Synthetic Biology, and TALEN ; Donor…TagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR-WT1shRNA
Plasmid#220560PurposeTo inducibly knockdown WT1 or EWSR1::WT1 expressionDepositorInsertWT1 shRNA (WT1 Human)
UseLentiviralTagsExpressionMutationPromoterTRE3G (TetOP) promoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJET-50-DExogron-mCherry-R11b
Plasmid#179904PurposeDonor with homologous arms for Rab11b to knock in DExogron (=minilAA7-DExCon) mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMKRQ_11_DExCon-mNeonGreen-R25
Plasmid#179896PurposeDonor with homologous arms for Rab25 to knock in DExCon-mNeonGreen moduleDepositorInsertRAB25, member RAS oncogene family (RAB25 Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-23-DExCon-mCherry-R11b
Plasmid#179898PurposeDonor with homologous arms for Rab11b to knock in DExCon-mCherry moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-67-DExCon-mNeonGreen-R11b
Plasmid#179899PurposeDonor with homologous arms for Rab11b to knock in DExCon-mNeonGreen moduleDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-59-DExCon-mTagBFP2-R25
Plasmid#179900PurposeDonor with homologous arms for Rab25 to knock in DExCon-mTagBFP2 moduleDepositorInsertRAB25, member RAS oncogene family (RAB25 Human)
UseCRISPR; Donor for homologous based knock inTagsmTagBFP2ExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only