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CBM9-(PT4)P

Plasmid

#171708

Purpose

Carries CBM9 fusion tag

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

Cellulose Bindind Protein 9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMP89b CvTA V124N

Plasmid

#141207

Purpose

Chromobacterium violaceum transaminase with enhances affinity for PLP. TEV cleaving site after the N-terminal Histag.

Depositor

Francesca Paradisi

Article

Roura Padrosa et al Front Bioeng Biotechnol. 2019 Oct 18;7:2

Insert

CvTA

Use

Tags

Expression

Bacterial

Mutation

V124N

Promoter

Available since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244

Plasmid

#17859

Depositor

David McKay

Article

Kwon et al J Mol Biol. 2003 Jul 4. 330(2):185-95.

Insert

Haemophilus influenzae heat shock loci U and V

Use

Tags

Expression

Bacterial

Mutation

coding sequence for residues 108-243 of HslU (the…

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

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pNCS mOrange

Plasmid

#91764

Purpose

Bacterial expression of a fluorescent protein, monomeric Orange (mOrange)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Orange

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS tdTomato

Plasmid

#91767

Purpose

Bacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

tandem dimer Tomato

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS mStrawberry

Plasmid

#91768

Purpose

Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Strawberry

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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FN207

Plasmid

#50497

Depositor

Kenneth Yamada

Article

Danen et al J Biol Chem. 1995 Sep 15;270(37):21612-8

Insert

NM_001306129.1 (FN1 Human)

Use

Tags

Expression

Bacterial

Mutation

repeats 6-10

Promoter

T7

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
CBM9-ID-F

Plasmid

#171639

Purpose

Expresses SARS-CoV-2 Spike epitope

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

SARS-CoV-2 Spike protein (S SARS-CoV-2)

Use

Tags

CBM-9

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CBM9-ID-H1

Plasmid

#171709

Purpose

Expresses SARS-CoV-2 Spike epitope

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

SARS-CoV-2 Spike protein (S SARS-CoV-2)

Use

Tags

CBM-9

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pNCS mCherry

Plasmid

#91769

Purpose

Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Cherry

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

See Depositor Comments

Promoter

T7

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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Bov.hsc70.RSET.a1-554.wt(NarI)

Plasmid

#12533

Depositor

David McKay

Article

Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

Insert

Bovine 70 kDa heat shock cognate protein fragment amino acids 1-554

Use

Tags

Expression

Bacterial

Mutation

deleted carboxy-terminal residues 555-650 of bovi…

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis).iGluSnFR

Plasmid

#41732

Purpose

Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Insert

GltI based Glutamate Biosensor

Use

Glutamate biosensor

Tags

Myc, PDGFR transmembrane helix (aa 513-561), and …

Expression

Mammalian

Mutation

cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

Promoter

CMV

Available since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only

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