-
Plasmid#173102PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP15 (PARP15 Human)
UseTagsmCeruleanExpressionBacterialMutationG287-N470, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pISceI-myl7:MBIC5-mCer3
Plasmid#74117PurposeZebrafish transgenic constructDepositorInsertMBIC5-mCer3
UseMrna generation for microinjection.TagsmCerulean 3ExpressionMammalianMutationPromoterSP6Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-kinase0
Plasmid#59122PurposeFAK fret control sensor without FERM domain with no linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
WasCFP /pQE-30
Plasmid#79858PurposeCyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.DepositorInsertWasCFP
UseTagsHisTagExpressionBacterialMutationCyan fluorescent protein mCerulean with substitut…PromoterT5 promoter / lac operatorAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM0
Plasmid#59125PurposeFAK fret control sensor without kinase domain with no linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-kinase10
Plasmid#59123PurposeFAK fret control sensor without FERM domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM10
Plasmid#59126PurposeFAK fret control sensor without kinase domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-Smad3
Plasmid#172098PurposeExpresses fluorescently tagged human Smad3 in mammalian cells.DepositorInsertSmad 3 (Smad3 Mouse)
UseTagsmNeonGreenExpressionMammalianMutationC49S mutation in mCeruleanPromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GDAP2-c005
Plasmid#173110PurposeExpression of recombinant fusion protein in E. coliDepositorInsertGDAP2 (GDAP2 Human)
UseTagsmCeruleanExpressionBacterialMutationM1-N231PromoterAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCer-4aa-VAMP2
Plasmid#53233PurposeFluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags4aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Stx1a-12aa-mCer
Plasmid#53236PurposeFluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.DepositorInsertSyntaxin 1A (Stx1a Rat)
UseTags12aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
-
RNF146-c005
Plasmid#173091PurposeExpression of recombinant fusion protein in E. coliDepositorInsertRNF146 (RNF146 Human)
UseTagsmCeruleanExpressionBacterialMutationR99-L183, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
P2-ECFP-pA (Construct 6)
Plasmid#55198PurposeSynthetic promoter responsive to gRNA2DepositorInsertmCerulean
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
CoV-NSP3-c001
Plasmid#173082PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep
UseTagsmCeruleanExpressionBacterialMutationE1000-K1173, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PH-dL5**
Plasmid#96946PurposePLC pleckstrin homology domain linked to FAP for CALI validationDepositorInsertPleckstrin homology domain
UseTagsdL5 and mCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ MBIC5-mCer3
Plasmid#74116PurposeFor generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)DepositorInsertMBIC5-mCer3
UseTransgenic constructTagsmCerulean 3ExpressionMutationPromoterMyl7 (Cmlc2)Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-IntCCCre-EF1a-Cerulean
Plasmid#160508PurposeExpresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertIntCCCre
UseCre/Lox and LentiviralTagsmCeruleanExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2022AvailabilityAcademic Institutions and Nonprofits only