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mEmerald-PCNA-19

Plasmid

#54221

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 (PCNA Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box

Plasmid

#112257

Purpose

Expresses RGS component of opto-RGS2 in mammalian cells

Depositor

Brian Chow

Article

Hannanta-Anan et al ACS Synth Biol. 2018 Jun 15;7(6):1488-14

Insert

CRY2-mCherry-(GGGGS)3-hRGS2box

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pPD_Pnpr-4::G-CaMP6s

Plasmid

#117423

Purpose

For Calcium imaging, expression of G-CaMP6 under the expression of a npr-4 promoter (AVA and RIV interneurons)

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

G-CaMP6s

Use

Tags

Expression

Worm

Mutation

Promoter

npr-4

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRS303 Bt δCOP

Plasmid

#73871

Purpose

Bos taurus Arcn1

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2 Promoter- COPD

Use

Tags

Expression

Yeast

Mutation

Promoter

Ret2 Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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3xFlag-HaloTag-SUZ12 HDR - pDY082

Plasmid

#171110

Purpose

Homology directed repair plasmid for generating HaloTag-SUZ12 cells

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

SUZ12 (SUZ12 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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DdMCU

Plasmid

#67642

Purpose

DdMCU-Flag in pACT2 vector human codon optimised

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

DdMCU

Use

Tags

Flag tag with linker

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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TU#1450_unc-53a_cDNA

Plasmid

#82361

Purpose

express unc-53a cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-53 a isoform (unc-53 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pPD_Pinx-1::G-CaMP3

Plasmid

#117422

Purpose

For Calcium imaging, expression of G-CaMP3 under the expression of a Pinx-1 promoter (AIB interneurons)

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

G-CaMP3

Use

Tags

Expression

Worm

Mutation

Promoter

inx-1

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pANY2

Plasmid

#112196

Purpose

This plasmid is used for efficient cloning of DNA and expression of protein in E. coli.

Depositor

Yingfeng An

Article

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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TU#1448_unc-73b_cDNA

Plasmid

#82362

Purpose

express unc-73b cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 b isoform (unc-73 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac1-bullfrog saxiphilin

Plasmid

#140595

Purpose

protein expression

Depositor

Dan Minor

Article

Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi:

Insert

saxiphilin bullfrog

Use

Tags

GFP-His10

Expression

Insect

Mutation

Deletion of M20 - please see depositor comment

Promoter

polyhedrin promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB511-TP-UnaG-FLAG

Plasmid

#203479

Purpose

For Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG-FLAG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB602-TP-mCherry-UnaG

Plasmid

#203483

Purpose

For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused mCherry-UnaG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSWD95Y14D

Plasmid

#159098

Purpose

CckA FRET Sensor with cyclic-di-GMP point mutant

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3

Use

Tags

Expression

Bacterial

Mutation

Mutated Y514 to D514

Promoter

Xylose

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPD_Pinx-1(1k + 50 bp)::Venus

Plasmid

#117421

Purpose

For analysis of AIB cell differentiation by tracking Venus fluorescence under the expression of Pinx-1 promoter

Depositor

Shohei Mitani

Article

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

Venus

Use

Tags

Expression

Worm

Mutation

Promoter

inx-1

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GCN4cc-Erp2CT

Plasmid

#73923

Purpose

GST fusion- coiled coil- Erp2 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp2 CT

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GCN4cc-Erp1CT

Plasmid

#73921

Purpose

GST fusion- coiled coil- Erp1 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp1 CT

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 δCLD+Ret2 2α

Plasmid

#73878

Purpose

Swapped helices

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

δCLD+Ret2 2α

Use

Tags

His

Expression

Yeast

Mutation

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD+δC2α

Plasmid

#73877

Purpose

Swapped helices

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD+δC2α

Use

Tags

His

Expression

Yeast

Mutation

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 CHI-15

Plasmid

#73884

Purpose

Chimaera 15

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

CHI-15

Use

Tags

His

Expression

Yeast

Mutation

Promoter

Met25

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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