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Plasmid#118597PurposeOne shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.DepositorInsertguide RNA against human CDK1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-pA-Tn5-Fl
Plasmid#124601PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase (Tnp) fusion protein
UseTagsProtein A and Tn5 transposase fusion protein with…ExpressionBacterialMutationPromoterT7Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPAGFP-C1
Plasmid#11910DepositorHas ServiceCloning Grade DNAInsertPAGFP
UseTagsExpressionMammalianMutationThese plasmids are essentially the same as the Cl…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPAGFP-N1
Plasmid#11909DepositorHas ServiceCloning Grade DNAInsertpPAGFP-N1
UseTagsExpressionMammalianMutationThese plasmids are essentially the same as the Cl…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJK503
Plasmid#155380PurposedCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceMay 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRTP801/TA-ODD
Plasmid#206212PurposeAn expression vector of hypoxia-responsive transactivator under control of RTP801 promoterDepositorInserttetracycline-responsive transactivator fused with oxygen-dependent degradation domain
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR
Plasmid#206213PurposePiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplificationDepositorInserttetracycline-responsive transactivator
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 3xFlag
Plasmid#110063PurposeFGFR2 expression in mammalian cells (JCOPCO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 F276C 3xFlag
Plasmid#110064PurposeFGFR2 F276C expression in mammalian cells (JCOPO paper)DepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Phenylalanine 276 to Cysteine (F276C)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 K41E 3xFlag
Plasmid#110105Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Lysine 41 to Glutamic acid (K41E)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-NAGK
Plasmid#23785DepositorInsertNAGK (NAGK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-FGFR2
Plasmid#23904DepositorInsertFGFR2 (FGFR2 Human)
UseGateway donor vectorTagsExpressionMutationA52D, T525A (compared to GenBank reference sequen…PromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-FN3K
Plasmid#23621DepositorInsertFN3K (FN3K Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-FN3KRP
Plasmid#23679DepositorInsertFN3KRP (FN3KRP Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-FGFR1
Plasmid#23922DepositorInsertFGFR1 (FGFR1 Human)
UseGateway donor vectorTagsExpressionMutationW211G and S351F compared to insert sequence provi…PromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCVD438
Plasmid#63759Purposeeae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.DepositorInserteae
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GNT5-blast
Plasmid#208380Purposelentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertBeta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationnt 922 c->g (PAM site inactivation)PromoterEF-1 alpha core promoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8
Plasmid#207031PurposeTet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7repeats)-GFP-Pho8
Plasmid#207011PurposeTet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-BAP1-C91S-HA
Plasmid#154021PurposeMammalian Expression of HA-tagged p.C91S Mutant BAP1DepositorInsertBAP1 (BAP1 Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationp.C91S (Missense Mutation, Catalytically Inactive)PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only