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Showing: 341 - 360 of 1456 results

  3. pJEP11-AAV-U6/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82705
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    82705-plasmid-map-sequence-id-170450

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  5. pB6-Hipp11-td-sfGFP-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183026
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-td-sfGFP-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183026-plasmid-map-sequence-id-360495

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  6. pB6-Hipp11-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183025
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183025-plasmid-map-sequence-id-360497

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  7. AiP11954 - pAAV-hsA2-AiE0078h_3xC2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1954)

    Plasmid
    #223968
    Purpose
    AiE0078h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in forebrain glutamatergic neurons
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223968-plasmid-map-sequence-id-450544

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  9. pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid
    #183027
    Purpose
    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    H2B-sfGFP-T2A-tdTomato
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183027-plasmid-map-sequence-id-360504

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  15. P33m

    Plasmid
    #120944
    Purpose
    MoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P11 from Mutalik library (medium)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120944-plasmid-map-sequence-id-245421

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  16. STK16

    Plasmid
    #39133
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STK16 (STK16 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. FDPS

    Plasmid
    #39131
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FDPS (FDPS Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. DHRS1

    Plasmid
    #39148
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DHRS1 (DHRS1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. wt Cus2p

    Plasmid
    #73046
    Purpose
    Wild-type S. cerevisiae Cus2p gene for recombinant expression and crystallization
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    T7
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    73046-plasmid-map-sequence-id-150790

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  20. L284F Cus2p

    Plasmid
    #73059
    Purpose
    S. cerevisiae Cus2p gene with L284F Mutation
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Leucine 284 to Phenylalanine
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    73059-plasmid-map-sequence-id-150795

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Showing: 341 - 360 of 1456 results