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Showing: 341 - 360 of 1330 results

  1. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid
    #191163
    Purpose
    Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    mCherry:s70587:EGFP-3xMS2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191163-plasmid-map-sequence-id-375409

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  13. pUC18 Apln5-HA-FlpO-pA-LoxP-PGK-Neo-pA-LoxP-Apln3 (SO89)

    Plasmid
    #159222
    Purpose
    Can be used to target the mouse Apln locus in mouse eggs or ES cells, in order to drive the mosaic expression of the protein HA-NLS-FlpO
    Depositor
    Rui Benedito
    Article
    Luo et al Nature. 2021 Jan;589(7842):437-441. doi:
    Insert
    Apln 5'-FlpO-WPRE-Sv40pA-LoxP-PGK-Neo-pA-LoxP-Apln
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Apln
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    159222-plasmid-map-sequence-id-315768

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  15. EW265 SFFV-mCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP (FLP-IN)

    Plasmid
    #236124
    Purpose
    Plasmid encoding fluorescent reporter for PUF-9R binding which expresses mCherry constitutively and EGFP upon stop codon deamination due to ADAR-PUF9R binding
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    mCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236124-plasmid-map-sequence-id-468460

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  16. EW267 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R-MARIAcomp(V241D, H243M) (FLP-IN)

    Plasmid
    #236127
    Purpose
    Plasmid encoding ADAR2dd attached to PUF-9R with deimmunizing mutations under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ADAR2dd-PUF-9R (ADARB1 Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E488Q, T501A in ADAR2dd; V241D, H243M in PUF-9R
    Promoter
    CMV promoter with two TetR binding sites
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236127-plasmid-map-sequence-id-468421

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  18. AiP15100: pAAV-AiE0140h_3xC2-minBG-FlpO-4X2C-WPRE3-BGHpA (Alias: CN5100)

    Plasmid
    #230873
    Purpose
    AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical and striatal Pvalb interneurons
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al bioRxiv [Preprint]. 2024 Sep 29:2024.09.
    Insert
    FlpO
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230873-plasmid-map-sequence-id-459485

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Showing: 341 - 360 of 1330 results