Addgene

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pcDNA3-Lyn-PlcR

Plasmid

#181830

Purpose

Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Use

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEX-CFP-hLC3WT

Plasmid

#24985

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEX-CFP-hLC3ΔG

Plasmid

#24986

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a mutant human MAP1-LC3B fused with ECFP (MAP1LC3B Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

deleted Gly at 121

Promoter

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Use

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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QUAS-jGCaMP7s-T2A-tdTomato

Plasmid

#200010

Purpose

QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

Depositor

Carolyn McBride

Article

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

3xP3-ECFP-jGCaMP7s-td-Tomato

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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mGDF8 donor CFP-hDF

Plasmid

#72590

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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hGDF8 donor CFP-hDF

Plasmid

#72591

Purpose

Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CFP

Plasmid

#13030

Purpose

Mammalian expression vector for cloning your gene fused to CFP

Depositor

Doug Golenbock

Has Service

Cloning Grade DNA

Insert

cyan fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCFP Vinculin

Plasmid

#50544

Depositor

Kenneth Yamada

Insert

Vinculin

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
CF-AIP

Plasmid

#61526

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with ECFP and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Use

Tags

AIP and ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
Cytosolic Fyn FRET Biosensor

Plasmid

#124654

Purpose

FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCFP Paxillin

Plasmid

#50510

Depositor

Kenneth Yamada

Insert

Paxillin (PXN Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pBullet-cyt-n

Plasmid

#53065

Purpose

destination vector with cytosol marker (ECFP) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBullet-cyt-c

Plasmid

#53066

Purpose

destination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBullet-er-n

Plasmid

#53067

Purpose

destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBullet-er-c

Plasmid

#53068

Purpose

destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ICUE4

Plasmid

#181846

Purpose

Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

ICUE4 (RAPGEF3 Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PDE4D2cat-ICUE4

Plasmid

#181847

Purpose

ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)

Use

Tags

ECFP, PDE4D2(86-418), and cpVenus(L194)

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-RIα-ICUE4

Plasmid

#181848

Purpose

ICUE4 cAMP sensor tethered to the PKA RIα subunit.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-ICUE4 (RAPGEF3, PRKAR1A Human)

Use

Tags

ECFP, PKA RIα, and cpVenus(L194)

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK204

Plasmid

#13534

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Use

Tags

See map

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge