Showing: 341 - 360 of 403 results
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Plasmid#164573PurposeIntegration of 2 LoxN sites in the genome of P. bergheiDepositorInsertsUseCre/LoxTagsExpressionMutationPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…AvailabilityAcademic Institutions and Nonprofits only
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pSLI-2xFKBP-GFP(1463000)
Plasmid#85789PurposeP. falciparum integration plasmid to fuse endogenous genes with tags using SLIDepositorInsertPF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
UseP. falciparum integrationTags2xFKBP, GFPExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pSLI-sandwich(0807600)
Plasmid#85790PurposeP. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLIDepositorInsertPF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
UseP. falciparum genomic integrationTags2xFKBP, GFPExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pSLI-TGD(1463000)
Plasmid#85791PurposeP. falciparum integration plasmid to disrupt genes using SLIDepositorInsertPF3D7146300 targeting region, GFP-2TA-Neo-R
UseP. falciparum genomic integration (gene disruptio…TagsGFPExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pSLI-N-GFP-2xFKBP-loxP(K13)
Plasmid#85792PurposeP. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLIDepositorInsertKelch13
UseCre/Lox; P. falciparum genomic integrationTags2xFKBP, GFPExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pBMN-dGBP1-TagBFP
Plasmid#80087PurposeMMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection markerDepositorInsertdGBP1-TagBFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SMT101
Plasmid#134815PurposeDual expression of DHFR and TYMS, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
SMT205
Plasmid#134817PurposeSMT201 with mutated RBS for DHFR, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailabilityAcademic Institutions and Nonprofits only -
SMT215
Plasmid#134818PurposeSMT205 with DHFR-FLAG-tag, western blotDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsFLAGExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-ZFP57
Plasmid#102716PurposeExpress DNA binding domain of human Zinc finger protein 57 in E. coliDepositorInsertZinc finger protein 57 (ZNF57 Human)
UseTagshisSUMOExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSLI-N-sandwich-loxP(K13)
Plasmid#85793PurposeP. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLIDepositorInsertKelch13
UseCre/Lox; P. falciparum genomic integrationTagsdouble 2xFKBP, GFPExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-CTCF(F1-F9)
Plasmid#102859PurposeExpress DNA binding domain of mouse CTCF in E. coliDepositorInsertCTCF (Ctcf Mouse)
UseTagshisSUMOExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
strep-IRF8
Plasmid#102872PurposeExpress strep-tagged mouse IRF8 protein in E. coliDepositorInsertIRF8 (Irf8 Mouse)
UseTagsstrep tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-Gli1
Plasmid#87225PurposeExpress hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coliDepositorInsertGLI1 (Gli1 Mouse)
UseTagshisSUMOExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-dCas13-APO1-YTH
Plasmid#204767PurposeGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)DepositorInsertdCas13-NLS-APO1-YTH-HA
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEf1alphaAvailabilityAcademic Institutions and Nonprofits only -
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2xFKBP-mCh-KDEL
Plasmid#173010PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.DepositorInsert2xFKBP-mCh-KDEL
UseTagsmCherryExpressionMammalianMutationPromoterChicken beta actin (CAG)AvailabilityAcademic Institutions and Nonprofits only -
2xFKBP-smFP(myc)-KDEL
Plasmid#173021PurposeFKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.DepositorInsert2xFKBP-smFP(myc)-KDEL
UseTagsFKBP and smFP(myc) non-fluorescentExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCSJ982
Plasmid#176622PurposeExpress yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertUb-MPGLW-Fbp1
UseTagsUbiquitin N-terminal fusion and triple flag tag C…ExpressionYeastMutationChanged first 4 N-terminal amino acid residues of…PromoterTDH3tc3AvailabilityAcademic Institutions and Nonprofits only
Showing: 341 - 360 of 403 results