Addgene

We narrowed to 403 results for: Argonaute

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psiCHECK2-let-7 4x

Plasmid

#20930

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Mar 4. ():.

Insert

4x let-7 target sites

Use

Luciferase

Tags

Expression

Insect and Mammalian

Mutation

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK2-let-7 2x

Plasmid

#20929

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Mar 4. ():.

Insert

2x let-7 target sites

Use

Luciferase

Tags

Expression

Insect and Mammalian

Mutation

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only
psiCHECK2-let-7 8x

Plasmid

#20931

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Mar 4. ():.

Insert

8x let-7 target sites

Use

Luciferase

Tags

Expression

Insect and Mammalian

Mutation

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK2-IRES-let-7 8x

Plasmid

#20932

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Mar 4. ():.

Insert

8x let-7 target sites

Use

Luciferase

Tags

Expression

Insect and Mammalian

Mutation

168bp IRES from 5'UTR of reaper gene in Dros…

Promoter

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2

Plasmid

#72207

Purpose

Mammalian expression of full-length human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Flag-Ago2-FDM3

Plasmid

#72210

Purpose

Mammalian expression of aa 227-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-226

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM1

Plasmid

#72208

Purpose

Mammalian expression of aa 1-372 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 373-860

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM2

Plasmid

#72209

Purpose

Mammalian expression of aa 1-517 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 518-860

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM4

Plasmid

#72211

Purpose

Mammalian expression of aa 373-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-372

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM5

Plasmid

#72212

Purpose

Mammalian expression of aa 518-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-517

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pIRESneo-FLAG/HA EYFP

Plasmid

#10825

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

EYFP

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-eGFP

Plasmid

#221710

Purpose

Expression of eGFP in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

eGFP

Use

Fish expression

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFM-NbrWT

Plasmid

#40830

Purpose

Expresses Flag, Myc tagged Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr (Nbr Fly)

Use

Tags

3xFLAG and 6xMyc

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
psiCheck-2-3 x mir-34

Plasmid

#40831

Purpose

Luciferase reporter

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

three sites partially complementary to miR-34 (mir-34 Fly)

Use

RNAi

Tags

luciferase

Expression

Insect

Mutation

Promoter

SV40

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+-drAgo2

Plasmid

#221711

Purpose

Expression of zebrafish Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFW-Ago1

Plasmid

#50553

Purpose

Expression of FLAG tagged Ago1 in S2 cell

Depositor

Yukihide Tomari

Article

Kawamata et al Nat Struct Mol Biol. 2009 Sep;16(9):953-

Insert

Argonaute1 (AGO1 Fly)

Use

Tags

FLAG

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr-D435A/E437A

Plasmid

#40829

Purpose

Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr D435A/E437A (Nbr Fly)

Use

Tags

3xFLAG and 6xMyc

Expression

Insect

Mutation

D435A/E437A, part of the DEDD motif; mutations p…

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG3-drAgo2D-A

Plasmid

#221734

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D683A in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2Y-F

Plasmid

#221732

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Y680F in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG3-drAgo2D-E

Plasmid

#221731

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E in active site

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge