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Anti-Frataxin [N191/7R]

Plasmid

#177511

Purpose

Mammalian Expression Plasmid of anti-Frataxin (Human). Derived from hybridoma N191/7.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Frataxin (Homo sapiens) recombinant mouse monoclonal antibody (FXN Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-Chantico

Plasmid

#163897

Purpose

Luciferase reporter for mouse Cxcl1 with Chantico promoter

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Cxcl1

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pChantico-Tdel-Firefly

Plasmid

#163902

Purpose

Luciferase reporter for Chantico lncRNA with Tbx5 site deletion

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Chantico with Tbx5 consensus binding site deletion

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pChantico-pAS-Firefly

Plasmid

#163903

Purpose

Luciferase reporter for Chantico lncRNA with triple pAS insertion downstream of the promoter

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Chantico

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Cyterm-mGreenLantern pDRF1-GW

Plasmid

#168091

Purpose

Expresses Cyterm-mGreenLantern to check for monomericity.

Depositor

Bas Teusink

Insert

mGreenLantern

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP N1 AURKA deltaNter

Plasmid

#157753

Purpose

Expression of AURKA without Nter, fused to GFP in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-TRIP8b [N212/7R]

Plasmid

#164154

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (Rat). Derived from hybridoma N212/7.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRIP8b (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-rUSP19ER-siRNAresistant

Plasmid

#155251

Purpose

for mammalian overexpression of the siRNA resistant ER isoform of rat USP19

Depositor

Simon Wing

Insert

USP19 (ER isoform) siRNA resistant (Usp19 Rat)

Use

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

Promoter

CMV

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-rUSP19CYT-siRNAresistant

Plasmid

#155252

Purpose

for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19

Depositor

Simon Wing

Insert

USP19 (CYT isoform) siRNA resistant (Usp19 Rat)

Use

Tags

6XHis-3XFlag

Expression

Mammalian

Mutation

Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …

Promoter

CMV

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_KARS_tRNA-anti-codon

Plasmid

#109865

Purpose

Protein expression and purification of KARS_tRNA-anti-codon

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

KARS_tRNA-anti-codon (KARS1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CANTsg

Plasmid

#154343

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CANT sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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Antares-SEPLuc C1A4E

Plasmid

#141088

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEP-C1A4E

Use

Tags

Expression

Mammalian

Mutation

Please see depositor comments

Promoter

CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Mad3 [N129A/6R]

Plasmid

#140075

Purpose

Mammalian Expression Plasmid of anti-Mad3 (Human). Derived from hybridoma N129A/6.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Mad3 (Human) recombinant mouse monoclonal antibody (MXD3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Antares-SEPLuc IRESv24

Plasmid

#141087

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEPLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-12 [N277/7R]

Plasmid

#114482

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse). Derived from hybridoma N277/7.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody (Syt12 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Nkx3-2_Anti-Sense

Plasmid

#124438

Purpose

Plasmid for anti-sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

NK3 homeobox 2 (Nkx3-2 Mouse)

Use

In situ probe

Tags

Expression

Mutation

Promoter

T7

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF4_CRISPR resistant

Plasmid

#122465

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF4 CRISPR resistant (SCAF4 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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PME-1_R369D mutant

Plasmid

#119294

Purpose

Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the PP2Ac binding motif

Depositor

Jukka Westermarck

Article

Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

Insert

PPME1 (PPME1 Human)

Use

Tags

Expression

Mammalian

Mutation

R369D

Promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Stonin-2 [N346/9R]

Plasmid

#114484

Purpose

Mammalian Expression Plasmid of anti-Stonin-2 (Human). Derived from hybridoma N346/9.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Stonin-2 (Homo sapiens) recombinant mouse monoclonal antibody (STON2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Clcn4 [N222/6R]

Plasmid

#114474

Purpose

Mammalian Expression Plasmid of anti-Clcn4 (Human). Derived from hybridoma N222/6.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Clcn4 (Homo sapiens) recombinant mouse monoclonal antibody (CLCN4 Mouse)

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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