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Plasmid#61891PurposePlasmid for run-off in vitro transcription of Saccharomyces paradoxus KBS RNADepositorInsertTelomerase RNA hairpin
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD04 S. arboricola telomerase RNA KBS
Plasmid#61893PurposePlasmid for run-off in vitro transcription of Saccharomyces arboricola KBS RNADepositorInsertTelomerase RNA hairpin
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRTagsExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41
Plasmid#213024PurposeExpresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusionDepositorInsertLINE-1 ORF2p (238-1061)
UseTagsHis6-MBP-3CExpressionBacterialMutationPromoterT7 / LacAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAP114
Plasmid#120799PurposeE. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)DepositorInsertPxyl::mCherryOpt
UseTagsExpressionBacterialMutation1.4 kb xylR-Pxyl DNA fragment from C. difficile R…PromoterPxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSW1728
Plasmid#120812PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)DepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRAN473
Plasmid#120813PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target proteinDepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRAN334
Plasmid#120814PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)DepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRAN357
Plasmid#120898PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target proteinDepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRT006.3 L1 dual-luciferase AI retrotransposition reporter
Plasmid#213031PurposeBi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integrationDepositorInsertTet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
UseLuciferaseTagsFirefly luciferase antisense intron (3' UTR)ExpressionMammalianMutationPromoterpTRE bidirectional tet-onAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV14-3X-Flag-SARS-CoV-2 S
Plasmid#145780PurposeOver expression of SARS-CoV-2 S (spike) in mammalian cell lineDepositorInsertSARS-CoV-2 S (S SARS CoV 2)
UseTags3xFlagExpressionMammalianMutationCodon optimized and C-terminal 19 amino acids del…PromoterCMVAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-SARS-CoV-2-S-RBD-8his
Plasmid#145145PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tagDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
UseTags8his tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-sfGFP
Plasmid#141184PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFPDepositorHas ServiceCloning Grade DNAInsertS surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
UseTagsSignal peptide from influenza HA and Superfolder …ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-rnSOX9 PQA/S (286-507)
Plasmid#62975PurposeExpresses HA-tagged rat SOX9 residues 286-507DepositorInsertSOX9 (Sox9 Rat)
UseTagsHA (YPYDVPDY)ExpressionMammalianMutationcodes for residues 286-507PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-Fc
Plasmid#141183PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-FcDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
UseTagsFc region of IgG1 (a.a. Asp-221 to Lys-447) and S…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5/G226A/A366S)
Plasmid#55797PurposeThis highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.DepositorInsertalpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T, G226A, A366S i…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D
Plasmid#169912PurposeExpression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.DepositorInsertdCjCas9
UseCRISPRTags10XGCN4, 2xSV40NLS, and SV40NLS-HAExpressionMammalianMutationD8A, H559A in cjCas9PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614G-trunc
Plasmid#158077PurposeExpresses SARS-CoV-2 spike protein with a D614G truncated mutationDepositorInsertSARS-CoV-2 spike (S )
UseTagsExpressionMammalianMutationD614GPromoterAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614-trunc
Plasmid#158076PurposeExpresses SARS-CoV-2 spike protein with a truncationDepositorInsertSARS-CoV-2 spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-GABA(A)R, Gamma2L/S [N452/73R]
Plasmid#177553PurposeMammalian Expression Plasmid of anti-GABA(A)R, Gamma2L/S (Human). Derived from hybridoma N452/73.DepositorInsertanti-GABA(A)R, Gamma2L/S (Homo sapiens) recombinant mouse monoclonal antibody (GABRG2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only