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Plasmid#33100DepositorInsertlats2 (Lats2 Mouse)
UseTagsHAExpressionMammalianMutationK655R, Kinase DeadPromoterCMVAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE32-pr-mEosFP (mEosFP-V69T)
Plasmid#99213PurposeBacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).DepositorInsertpr-mEosFP
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T for primed c…PromoterT5Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-Kaede (Kaede-A69T)
Plasmid#99228PurposeBacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).DepositorInsertpr-Kaede
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti CAG-FLAG-dCas9-VPR
Plasmid#114197PurposeLentivirus compatible dCas9-VPR construct driven by the CAG promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194283PurposeEF1a-dCas9-KRAB-GFP with Slc1a3 sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-NT sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194284PurposeEF1a-dCas9-KRAB-GFP with nontarget sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-TAOK2
Plasmid#172992Purposeconstitutive expression of FLAG-tagged TAOK2DepositorInsert3xFLAG-TAOK2 (TAOK2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synP-FLEX-splitTVA-EGFP-B19G
Plasmid#52473PurposeExpresses splitTVA-EGFP-B19GDepositorHas ServiceAAV1InsertTVA-P2A-EGFP-P2A-B19G
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterSynapsin-1Available sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
011LP-pAAV-TNT4-EmGFP-pA
Plasmid#120280PurposeAAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoterDepositorInsertEmGFP
UseAAVTags3XFlag and HAExpressionMutationPromoterTNT Cardiac Troponin T promoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRuby2-MACROD1
Plasmid#172587PurposeFor transient expression of C-terminal mRuby2-tagged MACROD1DepositorInsertMACROD1 (MACROD1 Human)
UseTagsmRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRuby2-MACROD1_d77
Plasmid#172588PurposeFor transient expression of C-terminal mRuby2-tagged MACROD1del1-77DepositorInsertMACROD1 (MACROD1 Human)
UseTagsmRuby2ExpressionMammalianMutationdeleted amino acids 1-76PromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-STK3
Plasmid#172990Purposeconstitutive expression of FLAG-tagged STK3DepositorInsert3xFLAG-STK3 (STK3 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3α-AS4+
Plasmid#184333PurposeExpression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorInsertNrxn3-alpha (Nrxn3 Mouse)
UseTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP11C-HA
Plasmid#209237PurposeMammalian expression of ATP11CDepositorInsertATP11C (ATP11C Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_12xTEAD_luc2
Plasmid#173004Purposefirefly luciferase reporter vector with 12x clustered TEAD binding sitesDepositorInsert12xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag Intersectin Long (human)
Plasmid#47393DepositorInsertintersectin long (ITSN1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His
Plasmid#83453PurposeFull-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)DepositorInsertpolycystin-1 (Pkd1 Mouse)
UseTags2xMyc, 3xFlag, and 6xHisExpressionMammalianMutationS4144DPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-TG
Plasmid#40026DepositorInsertsT (ATG)
beta-globin intron
Neo
GFP
diphteria toxin A
UseTagsExpressionMammalianMutationdeleted nucleotides 1-274 and insertion of FRT, …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-MOB1A
Plasmid#172988Purposeconstitutive expression of FLAG-tagged MOB1ADepositorInsert3xFLAG-MOB1A (MOB1A Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-GT
Plasmid#40025DepositorInsertsGFP
beta-globin intron
Neo
tdT-3Myc
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
Adeno EA
Plasmid#64071PurposeAdenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4DepositorInsertU6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
UseAdenoviralTagsFLAG-Cas9ExpressionMutationPromoterU6 and CBhAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1
Plasmid#39281DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1
Plasmid#39282DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-WWC1
Plasmid#172994Purposeconstitutive expression of FLAG-tagged WWC1DepositorInsert3xFLAG-WWC1 (WWC1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-IFT57-pcDNA3
Plasmid#47326PurposeExpresses FLAG epitope tagged IFT57 in mammalian cellsDepositorInsertIFT57 (Ift57 Mouse)
UseTagsFLAG epitopeExpressionMammalianMutationPromotercmvAvailable sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag Intersectin Short (human)
Plasmid#47392DepositorInsertIntersectin short (ITSN1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_TAOK3
Plasmid#209778Purposedoxycycline-inducible expression of TAOK3DepositorInsertTAOK3 (TAOK3 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZDonor-Nm-Cas9-gRNA-hU6
Plasmid#61366Purposeempty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNADepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.1 (mEosFP3.1-A69T)
Plasmid#99221PurposeBacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).DepositorInsertpr-mEos3.1
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_TAOK2
Plasmid#172979Purposedoxycycline-inducible expression of TAOK2DepositorInsertTAOK2 (TAOK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-A'Q-mito
Plasmid#158763PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-TAOK3
Plasmid#172993Purposeconstitutive expression of FLAG-tagged TAOK3DepositorInsert3xFLAG-TAOK3 (TAOK3 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-LZ-CD95L
Plasmid#104349PurposeExpresses human CD95L leucine zipper in mammalian cellsDepositorInsertCD95L leucine zipper (FASLG Human)
UseTagsoctahistidine tagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-STK4
Plasmid#172989Purposeconstitutive expression of FLAG-tagged STK4DepositorInsert3xFLAG-STK4 (STK4 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-TBP6.9F34MF37MF77M
Plasmid#168268PurposeExpresses human TBP6.9 with the F34M/F37M/F77M triple mutantDepositorInsertTBP6.9
UseTags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F34M, F37M and F77MPromoterT7 promotorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCT-GAL93-SFPQ
Plasmid#46320DepositorInsertSFPQ (SFPQ Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSP72-RBM8A-3'UTR AluY-Sz WT 2598-4066
Plasmid#175587Purposein vitro transcription of wt RBM8A 3'UTRDepositorInsertRBM8A-3'UTR AluY-Sz WT
UseIn vitro transcriptionTagsExpressionMutationPromoterT7Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124434PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in DrosophilaDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_TAOK1
Plasmid#209777Purposedoxycycline-inducible expression of TAOK1DepositorInsertTAOK1 (TAOK1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nV5-TAOK2
Plasmid#173000Purposeconstitutive expression of V5-tagged TAOK2DepositorInsertV5-TAOK2 (TAOK2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.2 (mEosFP3.2-A69T)
Plasmid#99224PurposeBacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).DepositorInsertpr-mEos3.2
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt-3HA
Plasmid#39284DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIK208
Plasmid#65630PurposesgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"DepositorInsertU6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-GFP
Plasmid#39290DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pEF NS34a-msGFP-'Q-mito
Plasmid#158764PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-3HA
Plasmid#39285DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceAug. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEA02
Plasmid#172193PurposeSuicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattL
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEA03
Plasmid#172194PurposeSuicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattR
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-EGFP-MACROD1
Plasmid#172584PurposeFor transient expression of N-terminal GFP-tagged MACROD1DepositorInsertMACROD1 (MACROD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only