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Showing: 851 - 900 of 2028 results

  1. PSMD3_BirA_Nterm

    Plasmid
    #221526
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Psmd3
    Depositor
    Alessandro Ori
    Insert
    PSMD3 (PSMD3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221526-plasmid-map-sequence-id-439227

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  2. PSMC3_BirA_Nterm

    Plasmid
    #221524
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Psmc3
    Depositor
    Alessandro Ori
    Insert
    PSMC3 (PSMC3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221524-plasmid-map-sequence-id-439226

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  3. PSMA4_BirA_Cterm

    Plasmid
    #221513
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Psma4
    Depositor
    Alessandro Ori
    Insert
    PSMA4 (PSMA4 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221513-plasmid-map-sequence-id-439223

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  4. pMX-Flag-GFP_TMEM88B

    Plasmid
    #227954
    Purpose
    Co-localisation experiment
    Depositor
    Alessandro Ori
    Insert
    TMEM88B
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    227954-plasmid-map-sequence-id-455413

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  7. pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

    Plasmid
    #113949
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-852
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113949-plasmid-map-sequence-id-224194

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  12. pcDNA3-HLA-cMyc-EcopT1R1

    Plasmid
    #113962
    Purpose
    mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R1 (TAS1R1 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    truncate N-terminal 24 residues
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113962-plasmid-map-sequence-id-223994

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  13. pCEP4-HA-Flag-natT1R2 ECD-MHC

    Plasmid
    #113957
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    residues 22-568 fused to a transmembrane tether
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113957-plasmid-map-sequence-id-215285

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  15. pCEP4-HA-Flag-natT1R2 FS1

    Plasmid
    #113955
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, one base pair dele…
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113955-plasmid-map-sequence-id-215169

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  16. pET-TRSter

    Plasmid
    #78865
    Purpose
    Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element
    Depositor
    Elias Arnér
    Article
    Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.
    Insert
    Thioredoxin Reductase (Txnrd1 Rat)
    Use
    Tags
    Mutated E. coli-type SECIS element (3’ end of ins…
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    78865-plasmid-map-sequence-id-147493

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  18. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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  20. pCEP4-HLA-c-myc-optiT1R3 ECD

    Plasmid
    #113960
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113960-plasmid-map-sequence-id-215290

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  21. pcDNA3.1(+)-HLA-cmyc-natT1R2

    Plasmid
    #113946
    Purpose
    mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 22-839
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113946-plasmid-map-sequence-id-224199

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  22. pcDNA3.1(+)-HLA-Flag-natT1R2

    Plasmid
    #113947
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from HLA class I …
    Expression
    Mammalian
    Mutation
    residues 22-839
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113947-plasmid-map-sequence-id-224107

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  23. pCEP4-HLA-cmyc-optiT1R3 a21-852

    Plasmid
    #113948
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-852
    Promoter
    CMV
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113948-plasmid-map-sequence-id-215162

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  26. pcDNA 3.3 HA-hTSPAN12

    Plasmid
    #115784
    Purpose
    Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.
    Depositor
    Harald Junge
    Article
    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.
    Insert
    TSPAN12 (TSPAN12 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    multiple silent mutations introduce restriction s…
    Promoter
    CMV
    Available since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    115784-plasmid-map-sequence-id-224023

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  27. ATP1A1_G4_Q118R_v2_Dual_epegRNA_tevopreQ1

    Plasmid
    #187456
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4_v2 optimized epegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R_v2 epegRNA (tevopreQ1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187456-plasmid-map-sequence-id-367464

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  31. pUC57-white[coffee]

    Plasmid
    #84006
    Purpose
    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4
    Depositor
    Phillip Zamore
    Article
    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.
    Insert
    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    A GC-to-AA mutation that creates a G589E missense…
    Promoter
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    84006-plasmid-map-sequence-id-165529

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  33. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

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  35. pMZ7

    Plasmid
    #223180
    Purpose
    pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.
    Depositor
    Paul Blainey
    Article
    Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.
    Type
    Empty backbone
    Use
    Chromosomal integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    223180-plasmid-map-sequence-id-441239

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  41. ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1

    Plasmid
    #187454
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187454-plasmid-map-sequence-id-367517

    Enlarge

  43. ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1

    Plasmid
    #187458
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N epegRNA (tevopreQ1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187458-plasmid-map-sequence-id-367467

    Enlarge

  45. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

    Plasmid
    #113953
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S, S212N, and …
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113953-plasmid-map-sequence-id-215167

    Enlarge

  46. pCEP4-HA-Flag-natT1R2 FS10

    Plasmid
    #113956
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, S212N substitution…
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113956-plasmid-map-sequence-id-215284

    Enlarge

  48. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

    Plasmid
    #113952
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S and S212N su…
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113952-plasmid-map-sequence-id-215166

    Enlarge

Showing: 851 - 900 of 2028 results