1. p426 25Q GPD

   Plasmid

   #1181

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 25Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. CFP(1-158)-gamma-11 in pcDNAI/Amp

   Plasmid

   #55620

   Purpose

   An amino-terminal CFP fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   CFP(1-158)-gamma-11 (GNG11 Bovine, Aequorea victoria)
   

   Use

   Tags

   CFP(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   Ggamma-11 was amplified by PCR, which added a Bam…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Cer(1-158)-gamma-11 in pcDNAI/Amp

   Plasmid

   #55628

   Purpose

   An N-terminal mCerulean fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   Cer(1-158)-gamma-11 (GNG11 Bovine, Aequorea victoria)
   

   Use

   Tags

   Cer(1-158) was fused to the amino terminus of Gga…

   Expression

   Mammalian

   Mutation

   Ggamma-11 was amplified by PCR, which added a Bam…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. makeTCR_Hs.TRAV39

   Plasmid

   #233978

   Purpose

   Hs TRAV gene for makeTCR assembly

   Depositor

   Michael Platten

   Insert

   TRAV39 (TRAV39 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. makeTCR_Hs.TRAV40

   Plasmid

   #233980

   Purpose

   Hs TRAV gene for makeTCR assembly

   Depositor

   Michael Platten

   Insert

   TRAV40 (TRAV40 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. makeTCR_Hs.TRAV34

   Plasmid

   #233973

   Purpose

   Hs TRAV gene for makeTCR assembly

   Depositor

   Michael Platten

   Insert

   TRAV34 (TRAV34 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. makeTCR_Hs.TRAV35

   Plasmid

   #233974

   Purpose

   Hs TRAV gene for makeTCR assembly

   Depositor

   Michael Platten

   Insert

   TRAV35 (TRAV35 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. makeTCR_Hs.TRAV38-1

   Plasmid

   #233976

   Purpose

   Hs TRAV gene for makeTCR assembly

   Depositor

   Michael Platten

   Insert

   TRAV38-1 (TRAV38-1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. makeTCR_Hs.TRAV26-2

   Plasmid

   #233968

   Purpose

   Hs TRAV gene for makeTCR assembly

   Depositor

   Michael Platten

   Insert

   TRAV26-2 (TRAV26-2 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. makeTCR_Hs.TRAV36/DV7

   Plasmid

   #233975

   Purpose

   Hs TRAV gene for makeTCR assembly

   Depositor

   Michael Platten

   Insert

   TRAV36/DV7 (TRAV36DV7 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. makeTCR_Hs.TRAV38-2/DV8

    Plasmid

    #233977

    Purpose

    Hs TRAV gene for makeTCR assembly

    Depositor

    Michael Platten

    Insert

    TRAV38-2/DV8 (TRAV38-2DV8 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. makeTCR_Hs.TRAV29/DV5

    Plasmid

    #233970

    Purpose

    Hs TRAV gene for makeTCR assembly

    Depositor

    Michael Platten

    Insert

    TRAV29/DV5 (TRAV29DV5 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBS ZfHoxb3a

    Plasmid

    #27522

    Depositor

    Victoria Prince

    Insert

    Hoxb3a (hoxb3a Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pLV-Enh eGFP Reporter-muKC2-X31

    Plasmid

    #59255

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    Krt80-ex5-7
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Mu Hsp68 minimal promoter

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pZBTB11-donor

    Plasmid

    #112348

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZBTB11 with GFP

    Depositor

    Kevin White

    Insert

    ZBTB11 homology arms flanking EGFP-IRES-Neo cassette (ZBTB11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:3:101651181:A>T

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0

    Plasmid

    #50022

    Purpose

    expresses Membrane tagged G-CaMP3 in cells expressing Cre

    Depositor

    David Anderson

    Article

    Vrontou et al Nature. 2013 Jan 31;493(7434):669-73. do

    Insert

    G-CaMP3
    

    Use

    AAV

    Tags

    MARCKS sequence (MGCCFSKT)

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVP91A-pcaHG

    Plasmid

    #113766

    Purpose

    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

    Depositor

    John Lipscomb

    Article

    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

    Inserts

    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    

    Use

    Tags

    Met-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HOXA7 (murine) His-tag pET

    Plasmid

    #8491

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA7 (Hoxa7 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG; makes protein by …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. GFP-mCGN

    Plasmid

    #236511

    Purpose

    For expression of GFP-Cingulin (mouse) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

    Insert

    CGN (Cgn Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEE028

    Plasmid

    #176785

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    305
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge