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Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI
Plasmid#116955PurposeExpresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-p62
Plasmid#199780PurposeUsed for the expression of mCherry labelled p62 (SMC Internal No. 391).DepositorInsertp62 (NUP62 Human)
UseTags6x His, TEV, mCherryExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET mRuby2 LIC cloning vector (u-mRuby2)
Plasmid#86932PurposepET LIC cloning vector for yORF-mRuby2DepositorTypeEmpty backboneUseTagsmRuby2ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET Flag TEV LIC cloning vector (2L-T)
Plasmid#29715DepositorTypeEmpty backboneUseTagsFLAG-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
Affibody HER2:243.BirA*-pET301/NT-DEST
Plasmid#174692PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
UseTagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Affibody EGFR:1907.BirA*-pET301/NT-DEST
Plasmid#174691PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
UseTagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His
Plasmid#171587PurposeBacterial expression of NLS-RfxCas13d-NLS 6HisDepositorInsertMKIEE-NLS-RfxCas13d-NLS-HA-6His
UseTagsHA-6HIS and MKIEEExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII TEV LIC cloning vector (2R-T)
Plasmid#29717DepositorTypeEmpty backboneUseTagsStrepII-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-GFP-Patronin CC 868–1457
Plasmid#59053Purposefor E. coli expression of Patronin CC truncationDepositorInsertPatronin (Patronin Fly)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CTLA4-FLN-ELP-His-ybbr
Plasmid#168038PurposeExpresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertCytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
UseTags3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET-(pos36)GFP-9xGGS-Cre-6xHis
Plasmid#62938PurposeExpression of (+36)GFP-GGS9-Cre-6xHis in bacterial cellsDepositorInsert(ps36)GFP-9xGGS-Cre
UseTags(pos36)GFP and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-OPTN WT
Plasmid#190191PurposePlasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797DepositorInsertoptineurin (OPTN Human)
UseTags6xHis, TEV, and mCherryExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET mRuby2 LIC cloning vector (H6-mRuby2)
Plasmid#86934PurposepET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2DepositorTypeEmpty backboneUseTagsBiotin, His6, Tev Recognition Sequence, and mRuby…ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET tdTomato LIC cloning vector (H6-tdTomato)
Plasmid#86928PurposepET LIC cloning vector for Biotin-His6-tev-yORF-tdTomatoDepositorTypeEmpty backboneUseTagsBiotin, His6, Tev Recognition Sequence, and tdTom…ExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-GST-eIF4G1-His6, eIF4E
Plasmid#37232DepositorUseTagsGST and His6ExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)
Plasmid#182395PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusionsDepositorInsertsVNp-LZ-VenusN154
VNp-LZ-VenusC155
UseTagsVesicle Nucleating peptide (VNp) & Leucine Zi…ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-SpCas9-HF1-plus-NLS-6xHis
Plasmid#207381PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSpCas9-HF1-plus
UseCRISPRTags6xHisExpressionBacterialMutationN497A, Q695A, Q926A, amino acids 1005-1013 replac…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FabF (KASII) pet28 N-terminal His Tag
Plasmid#75017PurposeExpresses the ketosynthase FabF/KASII from the E. coli fatty acid synthaseDepositorInsertFabF (fabF E. coli)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only