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Plasmid#89451PurposeExpression of GFP-tagged human Rab27B GG in mammalian cellsDepositorInserthuman Rab27B GG (RAB27B Human)
UseTagseGFPExpressionMammalianMutationGG (geranyl geranyl site is mutated)PromoterCMVAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-hTRF1-tagRFP-T
Plasmid#103811PurposeBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGLOW39
Plasmid#173068PurposemScarlet^SEC^3xMyc vector with ccdB sites for cloning homology armsDepositorInsertmScarlet-I-C1^SEC^3xMyc
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized mScarletExpressionWormMutationPromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP12-5ptpase enzyme
Plasmid#67515PurposeRecruitable human type IV 5-phosphatase enzymeDepositorUseTagsmRFPExpressionMammalianMutationINPP5E has C641A to destroy the C-terminal CAAX d…PromoterCMVAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hLaminB1
Plasmid#103800PurposeBLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hTRF1
Plasmid#103801PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-hPMCA2xb
Plasmid#47751Purposemammalian expression of mCherry tagged hPMCA2, xb splice variantDepositorInsertPMCA2x/b (ATP2B2 Human)
UseTagsmCherryExpressionMammalianMutationxb splice variantPromoterCMVAvailable sinceNov. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
mApple-Actin-C-18
Plasmid#54863PurposeLocalization: Actin, Excitation: 568, Emission: 592DepositorInsertActin (ACTB Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFP-plk1-AKAP
Plasmid#132967PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorUseTagsExpressionMammalianMutationC terminus of full-length Plk1 fused to the peric…PromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Clover-LaminA-C-18
Plasmid#56370PurposeLocalization: Nuclear Envelope, Excitation: 505, Emission: 515DepositorInsertLaminA (LMNA Human)
UseTagsCloverExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Mouse DsRed-Monomer CASP9 C325A Mutant
Plasmid#198401PurposeExpresses Mouse DsRed-Monomer CASP9 C325A Mutant Fusion Protein in Mammalian CellsDepositorInsertMouse Caspase-9 C325A Mutant (Casp9 Mouse)
UseTagsDsRed-monomerExpressionMammalianMutationChanged Cysteine 325 to Alanine to eliminate prot…PromoterCMVAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
FusionRed-Caveolin-C-10
Plasmid#56103PurposeLocalization: Membrane, Excitation: 580, Emission: 608DepositorInsertCaveolin (CAV1 Human)
UseTagsFusionRedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
FusionRed-CD36-C-10
Plasmid#56104PurposeLocalization: Membrane, Excitation: 580, Emission: 608DepositorInsertCD36 (CD36 Human)
UseTagsFusionRedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCIBN-LacI-tagRFP-T
Plasmid#103810Purpose'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 N-t
Plasmid#89980PurposeExpresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3DepositorInsertataxin-3 (ATXN3 Human)
UseTagsGFPExpressionMammalianMutationconstruct contains aa 1-182; deleted aa 183-361PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)3x
Plasmid#112057PurposeACTB mRNA tagged with 3 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation3 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)2x
Plasmid#112056PurposeACTB mRNA tagged with 2 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation2 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 C-t
Plasmid#89981PurposeExpresses the GFP-tagged C-terminus of ataxin-3DepositorInsertataxin-3 (ATXN3 Human)
UseTagsGFPExpressionMammalianMutationdeleted amino acids 1-182PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Tom20-mChF-AIP(TE)
Plasmid#61522PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TE mutation (see comments), Tom20, and m…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only