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Tet-O-FUW-NR3C1-P2A-mCherry

Plasmid

#203854

Purpose

Tet-inducible lentiviral plasmid expressing NR3C1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR3C1 (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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PTRF/Cavin1_L (OZ513)

Plasmid

#27188

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA216-CoV2-RBD-beta variant

Plasmid

#194598

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

Depositor

Ben Murrell

Article

Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

Insert

CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-TMEM165_STOP

Plasmid

#161438

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

TMEM165 (TMEM165 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-FRA1

Plasmid

#188665

Purpose

Expresses murine FRA1 (Fosl1)

Depositor

Günter Schneider

Article

Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

Insert

Fosl1 (Fosl1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_016-sgATF4-3

Plasmid

#202456

Purpose

Expression of a guide RNA targeting ATF4

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

ATF4 gRNA (ATF4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-ND-Cdt1-HA-Puro

Plasmid

#193767

Purpose

Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal HA tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

ND-Cdt1-HA (CDT1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

Promoter

TRE

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 ∆cap/∆bund

Plasmid

#74891

Purpose

mammalian expression of the capping and bundling activity mutant Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

V689D, L693D, L757A, K759A

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pMSCV40-EF1a-hBCL10-SO

Plasmid

#75256

Purpose

retroviral expression vector for expression of StrepOne-Tagged human BCL10

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

Bcl10 (BCL10 Human)

Use

Retroviral

Tags

StrepOne Tag

Expression

Mammalian

Mutation

Promoter

pMSCV-LTRs, EF1a

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75244

Purpose

CRISPR/Cas9 plasmid against human Helios

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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PB-iRFP-STOP-ReNL

Plasmid

#113965

Purpose

Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Inserts

iRFP670
ReNL

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

CMV - SV40-PolyA - SV40-PolyA and EF1A

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shDnd1-No7

Plasmid

#70068

Purpose

TRC lentiviral vector for shRNA against mouse Dnd1 #7

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (Dnd1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only
pMSCV40-EF1a-hBCL10(1-185)-SO

Plasmid

#75258

Purpose

retroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-185)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

Bcl10 (BCL10 Human)

Use

Retroviral

Tags

StrepOne Tag

Expression

Mammalian

Mutation

AS 1-185 (w/o C-terminus)

Promoter

pMSCV-LTRs, EF1a

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pitx2_R (OZ534)

Plasmid

#27211

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pitx2 (pitx2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pitx2_L (OZ533)

Plasmid

#27210

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pitx2 (pitx2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
yap1_R (OZ590)

Plasmid

#35230

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting yap1 (yap1 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
yap1_L (OZ589)

Plasmid

#35229

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting yap1 (yap1 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
NFATc1_L (OZ543)

Plasmid

#28068

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting NFATc1 (nfatc1 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
NFATc1_R (OZ544)

Plasmid

#28069

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting NFATc1 (nfatc1 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
histamine receptor H3 (hrh3)_R (OZ550)

Plasmid

#28075

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting histamine receptor H3 (hrh3) (hrh3 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only