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pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159833

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-535)

Plasmid

#135439

Purpose

Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-N-SH2

Plasmid

#111274

Purpose

express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)

Use

Tags

HHHHHHENLYFQG

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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p35S::SISOBIR1-YFPc

Plasmid

#86164

Purpose

SOBIR1 split YFP

Depositor

Silke Robatzek

Article

Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

Insert

SOBIR1 (SOBIR1 Mustard Weed)

Use

Tags

YFPc

Expression

Plant

Mutation

Promoter

35S

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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p35S::AtSOBIR1-YFPn

Plasmid

#86165

Purpose

SOBIR1 split YFP

Depositor

Silke Robatzek

Article

Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

Insert

SOBIR1 (SOBIR1 Mustard Weed)

Use

Tags

YFPn

Expression

Plant

Mutation

Promoter

35S

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-TGAT(C242S,C253S)

Plasmid

#84336

Purpose

Expresses TGAT tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

TGAT(C242S,C253S) (TRIO Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

C242S, C253S

Promoter

CMV

Available since

Dec. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase delta 55

Plasmid

#59824

Purpose

Allows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

delta 55

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-XBP1 mNeonGreen NLS

Plasmid

#115968

Purpose

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

X-box binding protein 1 (XBP1 Human, Synthetic)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCAX APP 695

Plasmid

#30137

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Use

Tags

Expression

Mammalian

Mutation

wild type splice variant 695

Promoter

Available since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-SRRM2-mCherry

Plasmid

#174089

Purpose

Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

Depositor

Katie Ullman, Wesley Sundquist

Article

Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

Insert

SRRM2 (SRRM2 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TREtight

Available since

Sept. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.SFFV.tRFP

Plasmid

#57826

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-tRFP

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCAX FLAG APP

Plasmid

#30154

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

wild type splice variant 751

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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hSynapsin-PE2-Cterminal-lenti

Plasmid

#135956

Purpose

Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) C-terminal

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

MMLV RT(D200N, T306K, W313F, T330P, L603W)

Promoter

hSynapsin

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAX APP 751

Plasmid

#30138

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Use

Tags

Expression

Mammalian

Mutation

wild type splice variant 751

Promoter

Available since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A2

Plasmid

#31100

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF4A (HNF4A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

splice variant 2

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pSL5069 (pDonor(AAVS1),CRISPR_Pse)

Plasmid

#200904

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des13

Plasmid

#110213

Purpose

Yeast Surface Display of G6des13

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des13

Use

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

Promoter

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des1

Plasmid

#110212

Purpose

Yeast Surface Display of G6des1

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des1

Use

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pmGFP11C-Tau

Plasmid

#71434

Purpose

Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433

Depositor

Henri Huttunen

Article

Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

Insert

Tau (0N4R) (MAPT Human)

Use

Tags

GFP (213-228)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only