We narrowed to 26,318 results for: sta

1. hCCR5-LSSmOrange

   Plasmid

   #110194

   Purpose

   Encodes for human CCR5 coding sequence tagged with the LSS-mOrange FP

   Depositor

   Sergi Padilla Parra

   Article

   Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

   Insert

   hCCR5 (CCR5 Human)
   

   Use

   Tags

   LSSmOrange

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES-Puro-MGMT

   Plasmid

   #187654

   Purpose

   Expresses hMGMT together with the puromycin resistance gene via an IRES.

   Depositor

   Robert Sobol

   Article

   Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

   Insert

   MGMT (MGMT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK1E-RNAP

   Plasmid

   #48144

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase K1E
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNAintron-CHIKV-Structural polyprotein

   Plasmid

   #215698

   Purpose

   Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV structural polyprotein Capsid-E3-E2-6K-E1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. 145-pGL3ctrl-3' UTR

   Plasmid

   #28175

   Depositor

   Michael Kastan

   Article

   Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

   Insert

   Luciferase flanked by tp53 UTRs (TP53 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Note that insert length is based on sum of the 2 …

   Promoter

   Available since

   March 15, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pOTTC841 - pAAV minP secNLuc

   Plasmid

   #82498

   Purpose

   Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

   Depositor

   Brandon Harvey

   Article

   Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

   Insert

   secNLuc
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   c-Fos minimal promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

   Plasmid

   #52521

   Purpose

   Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

   Depositor

   Scott Sternson

   Article

   Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

   Insert

   ChR2-2a-hM4D
   

   Use

   AAV and Cre/Lox

   Tags

   2xHA on ChR2

   Expression

   Mutation

   Promoter

   CBA

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

   Plasmid

   #128404

   Purpose

   Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Exotoxin A (toxA P. aeruginosa)
   

   Use

   Tags

   6xHis tag and DsbA signal sequence for periplasmi…

   Expression

   Bacterial

   Mutation

   242DNNNS246 and 384DQNRT388 engineered glycosylat…

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. eGFP-SUN1

   Plasmid

   #213515

   Purpose

   Expresses human eGFP-SUN1 in mammalian cells

   Depositor

   Karim Mekhail

   Article

   Shokrollahi et al bioRxiv 2023.05.07.539750

   Insert

   SUN1 (SUN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SUN1 has a silent mutation on its gene (nucleotid…

   Promoter

   CMV

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTrc99S-ssDsbA-TTc-4xDQNAT

    Plasmid

    #128401

    Purpose

    Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin fragment C domain
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid

    #128402

    Purpose

    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin light chain domain
    

    Use

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating E234A mutation

    Promoter

    trc

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDIRECT_26H

    Plasmid

    #91150

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMP71-mIL15-IL12rb1-sushi-il12rb2

    Plasmid

    #174606

    Purpose

    Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    Expresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCosSAD1AprR

    Plasmid

    #190786

    Purpose

    An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination

    Depositor

    Jan Kormanec

    Article

    Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

    Inserts

    upstream region of the aur1 cluster for auricin
    ampramycin resistance cassette
    downstream region of the aur1 cluster for auricin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HOXB8 (murine) HIS-tag pET

    Plasmid

    #8540

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB8 (Hoxb8 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    starts at amino acid #2 (Ser)

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

16. iRFP713-N2-Frankenbody-Flag

    Plasmid

    #175291

    Purpose

    Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with iRFP713
    

    Use

    Tags

    iRFP713

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCDH-EFS-FlpO

    Plasmid

    #191509

    Purpose

    Lentiviral Flp Recombinase driven by the EFS promoter

    Depositor

    Eric Snyder

    Article

    Orstad et al Dev Cell. 2022 Aug 8;57(15):1866-1882.e1

    Insert

    flp recombinase (flp )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/EFS promoter inserted

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pS848∙CsR

    Plasmid

    #199240

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PEM7

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR1A DHFR.cHSF1

    Plasmid

    #134737

    Purpose

    Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

    Depositor

    Matthew D. Shoulders

    Article

    Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

    Insert

    DHFR.cHSF1 (HSF1 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    DHFR

    Expression

    Mutation

    Deletion of amino acids 186-202

    Promoter

    None

    Available since

    Oct. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNAintron-CHIKV-nsp1-c3xFLAG

    Plasmid

    #215694

    Purpose

    Produces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV nsp1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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