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pTETDuet_M42FDHFR_R127ATS

Plasmid

#191302

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

M42F DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_G121VDHFR_R127ATS

Plasmid

#191303

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

G121V DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YG121VDHFR_R127ATS

Plasmid

#191304

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

F31Y/G121V DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FG121VDHFR_R127ATS

Plasmid

#191305

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

M42F/G121V DHFR, R127A TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_D27NDHFR_WTTS

Plasmid

#191283

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

D27N DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FDHFR_WTTS

Plasmid

#191284

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

M42F DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_G121VDHFR_WTTS

Plasmid

#191285

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

G121V DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FG121VDHFR_WTTS

Plasmid

#191286

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

M42F/G121V DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_F31YG121VDHFR_WTTS

Plasmid

#191287

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

F31Y/G121V DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_WTDHFR_Q33STS

Plasmid

#191288

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

WT DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_M42FDHFR_Q33STS

Plasmid

#191290

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

M42F DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTETDuet_G121VDHFR_Q33STS

Plasmid

#191291

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Use

Tags

Expression

Bacterial

Mutation

G121V DHFR, Q33S TYMS

Promoter

T7 (folA), Tet (thyA)

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-MCS1-GB1_AB

Plasmid

#148370

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-MCS2-GB1_AB

Plasmid

#148371

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP1D5

Plasmid

#165063

Purpose

Characterisation of yeast promoter using yEGFP as reporter gene and PGK1 terminator

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA3998rc

Plasmid

#184851

Purpose

A retroviral vector with blasticidin resistance for cloning inserts between loxP sites

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA5132

Plasmid

#184858

Purpose

Retroviral vector, hygromycin resistance hTFAM expression

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

TFAM human

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN513

Plasmid

#175365

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, HIS3-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN514

Plasmid

#175366

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, TRP1-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN515

Plasmid

#175367

Purpose

Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, LEU2-selected.

Depositor

Daniel Nickerson

Article

Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge