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Plasmid#111895PurposessAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPREDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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pEMS1964
Plasmid#82574PurposeHigh copy number plasmid containing Ple305 MiniPromoter insert.DepositorInsertPle305
UsePleiades promoter project [sic, pleaides plieades]TagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB3053(gRNA X-2, XI-5, XII-4)
Plasmid#73295PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19 PKC epsilon
Plasmid#8442DepositorInsertMouse Protein Kinase C epsilon (Prkce Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
RF-211-UB-Lyn
Plasmid#79184PurposeExpression of the C-terminal fragment of Lyn kinase that is endogenously generated by caspase cleavage in mammalian cellsDepositorInsertLyn (LYN Human)
UseTags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-18PromoterCMVAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 1917
Plasmid#53024PurposeAmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1DepositorInsertsUseSynthetic BiologyTagsAsp terminator, RBS(st7), and T1 terminatorExpressionBacterialMutationPromoterpCon and pLlacO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKP136 (pRS406-PRC1p-PRC1-PA-ADH1t)
Plasmid#106471PurposeExpresses Prc1 with a C-terminal PA tag in yeast cellsDepositorInsertPRC1
UseTagsPAExpressionBacterial and YeastMutationPromoterPRC1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB3038(XII-1 MarkerFree)
Plasmid#73278PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3040(XII-4 MarkerFree)
Plasmid#73280PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSK21_HUMAN_D0
Plasmid#79707PurposeThis plasmid encodes the kinase domain of CSK21. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertCSK21
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
3604 pGEX4T-1 Bad S136A (104-141)
Plasmid#8801DepositorInsertBad (Bad Mouse)
UseTagsGSTExpressionBacterialMutationContains AA's 104-141; S136APromoterPtacAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCfB3037(XI-5 MarkerFree)
Plasmid#73277PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4 WW2
Plasmid#111882PurposeMode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: NEDD4 WW2 fused to C-termin…ExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS1212
Plasmid#63785PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection markerDepositorInserteGFP-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseGFP-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
KAPCA_HUMAN_D0
Plasmid#79741PurposeThis plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKAPCA
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPD95.75-cYAP-1
Plasmid#66857PurposeGenerate transgenic C.elegans with cYAP-1 fused to GFPDepositorInsertC.elegans YAP-1 (yap-1 Nematode)
UseTagsGFPExpressionWormMutationSerine104 is mutated to alaninePromoterC. elegans 5'UTRAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD95.75-cYAP-1 S104A
Plasmid#66858PurposeGenerate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanineDepositorInsertYAP-1 (yap-1 Nematode)
UseTagsGFPExpressionWormMutationSerine104 is mutated to alaninePromoterAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Diap3 in situ probe
Plasmid#45602DepositorInsertDiap3 in situ probe (Diaph3 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 1589-2321 of BC028920PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Crim1 in situ probe
Plasmid#45600DepositorInsertCrim1 in situ probe (Crim1 Mouse)
UseIn situTagsExpressionMutationinsert contains bp#4062-4502 of NM_015800.3PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only